“青大一号”紫花苜蓿叶黄素提取研究  

Study on the Extraction of Lutein from Medicago sativa L. cv. Qingda 1

作　　者：畅喜云[1,2] 杨国柱[3] 

机构地区：[1]青海畜牧兽医职业技术学院,青海湟源812100 [2]甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [3]青海大学,青海西宁810000

出　　处：《安徽农业科学》2013年第11期4855-4857,共3页Journal of Anhui Agricultural Sciences

基　　金：青海省科技攻关项目(2005-N-103);青海省自然科学基金(2012-Z-941Q)

摘　　要：[目的]从紫花苜蓿中提取叶黄素。[方法]以"青大一号"紫花苜蓿为材料,采取有机溶剂蒸馏萃取法,通过设置单因素提取剂(配比)、提取温度、料液比来考察对叶黄素提取工艺的影响。同时通过L9(34)正交试验来确定"青大一号"紫花苜蓿的最佳提取条件。[结果]提取叶黄素的最佳工艺条件为:选取乙醇和丙酮为混合提取剂,体积比2:1,料液比1.2∶50,提取温度55℃,此条件下提取叶黄素的含量为1.453 mg/L。[结论]从紫花苜蓿中提取叶黄素具有可行性。[Objective] The research aimed to extract lutein from alfalfa effectively,[Method] Using Medicago sativa L.cv.Qingda 1 as materials,single factor extraction solvent（ratio）,extraction temperature,material-liquid ratio were set up to study their effects on the extraction technology of lutein.The optimum extraction conditions of M.sativa L.cv.Qingda 1 were confirmed by using L9（34） orthogonal experiment.[Result] The optimum technology conditions for extracting lutein were as follows： selecting ethanol and acetone as mixed extraction solvent,volume ratio of 2∶1,material-liquid ratio of 1.2∶50,extraction temperature of 55 ℃.Under these conditions,lutein content was 1.453 mg/L.[Conclusion] It was feasible to extract lutein from alfalfa.

关 键 词：紫花苜蓿“青大一号” 叶黄素 提取 

分 类 号：S541.1[农业科学—作物学]