VEGF在喉癌中的表达及临床意义  

Expression and clinical significance of VEGF in laryngeal carcinoma

作　　者：鲁赛帅[1] 倪昊生[2] 刘浩[2] 章建国[3] 刘益飞[3] 

机构地区：[1]南通大学附属医院ICU,南通226001 [2]南通大学附属医院耳鼻喉科,南通226001 [3]南通大学附属医院病理科,南通226001

出　　处：《南通大学学报（医学版）》2013年第3期179-181,共3页Journal of Nantong University(Medical sciences)

基　　金：南通市社会事业科技创新与示范计划项目(HS2011042)

摘　　要：目的 :检测血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)在喉癌组织中的表达,探讨其与喉癌生物学行为及临床病理学特征间的关系。方法:采用免疫组织化学S-P法,检测84例喉癌、5例不典型增生及5例声带息肉组织中VEGF表达。用统计学方法分析其表达与喉癌临床病理因素的关系及相关性。结果:VEGF蛋白定位于肿瘤细胞胞质和肿瘤间质血管内皮。VEGF总阳性率为70.2%。不同分化程度喉癌间的表达有差异;不同分期喉癌间的表达有差异;有淋巴结转移组和无淋巴结转移组组间差异有统计学意义。结论:喉癌细胞存在VEGF的自分泌,VEGF过表达能直接刺激肿瘤细胞增殖。VEGF的表达水平随肿瘤分化程度的降低、临床分期的由早变晚以及淋巴结转移的出现而增高,提示其可作为喉癌恶性程度增高的标志。Objective： To detect the expressions of vascular endothelial growth factor（VEGF） in laryngeal carcinoma and investigate its possible relationship with biological behavior and clinicopathological characteristics in laryngeal carcinoma.Methods： S-P immunohistochemical staining was used to examine the expressions of VEGF in 84 laryngeal carcinoma and 5 vocal cords polyp（VCP） and 5 atypical hyperplasia tissue.The relationships between the expression of VEGF in laryngeal carcinoma and clinical pathologic characters was analyzed by statistical methods.Results： VEGF was localized in cell plasma and vascular endothelial cells of laryngeal carcinoma.The total expression of VEGF was 70.2% in laryngeal carcinoma.The expression had a difference between differentiated degree of laryngeal carcinoma.And there was significiant difference in the expression of different stage.The rate of expression of VEGF in tissues with and without lymphatic metastasis was different,which was significant.Conclusions： Laryngeal carcinoma cells have the autocrine function of VEGF.Overexpression of VEGF can directly stimulated the proliferation of tumor cells.The increase of the expression level of VEGF was related with the lower differentiation of tumors,later clinical stage and occurrence of lymph node metastasis,which suggests that VEGF may be the marker of the increase of malignancy grade in laryngeal carcinoma.

关 键 词：喉癌 血管内皮细胞生长因子 免疫组织化学S-P法 

分 类 号：R739.65[医药卫生—肿瘤]