应用环孢菌素A制备BALB/C小鼠肺癌模型的实验研究  

An experimental study on preparation of BALB/C mice lung cancer model using cyclosporin A

作　　者：吴洁[1] 唐静[1] 谢伟敏[2] 王洪学[2] 陆永奎[2] 周文献[2] 

机构地区：[1]广西医科大学研究生学院,广西南宁530021 [2]广西医科大学附属肿瘤医院,广西南宁530021

出　　处：《右江民族医学院学报》2013年第3期268-270,共3页Journal of Youjiang Medical University for Nationalities

基　　金：广西自然科学基金资助项目(编号:桂科回0575019)

摘　　要：目的探讨环孢菌素A(CsA)对建立皮下肺腺癌异种移植的BALB/C小鼠模型成瘤率的影响。方法取50只BALB/C小鼠腋窝皮下接种人肺腺癌细胞系A549后,随机分为实验组和对照组各25只。实验组于接种第24h起每天腹腔注射CsA,连续10天。对照组则给予腹腔注射同体积的生理盐水。比较两组的成瘤率以及胸腺、脾脏重量指数、CD4+、CD8+T移植瘤免疫组化细胞数。结果实验组移植成瘤率为88.00%,显著高于对照组的52.00%(P<0.05)。与对照组相比,实验组胸腺指数显著降低(P<0.05),CD4+、CD8+T细胞数以及CD4+/CD8+T细胞比值亦显著降低(P均<0.01),但脾脏指数差异无显著性(P>0.05)。结论使用CsA可以提高人肺腺癌细胞系A549制备皮下异种移植的BALB/C小鼠肺癌模型的成功率。Objective To study the effect of eyclosporin A （CsA） on tumorigenesis el established by subcutaneous xenotransplantation of lung adenocarcinoma. Methods of BALB/C mice mod Human lung adeno carcinoma cell A549 was used to establish mice model, 50 BALB/C mice were randomly divided into experimental group （ n =25） and the control group （ n =25）. Experimental group received intraperitoneal injection of CsA with 25 mg/kg for 10 days continuously, and control group received intraperitoneal injection of equal dose of physiological saline. Tumor formation rate, thymus weight index, spleen weight index and peripheral blood CD4＋ , CD8＋ T cell subpopulation percentage were compared between the two groups. Results The tumor formation rate of experimental group was significantly higher than that of control group （88.00% vs. 52.00%, P〈0.05）. Compared with control group, thymus index of experimental group were significantly de- creased （P 〈0.05）, CD4＋ and CD8＋ T cell subpopulation percentage, and ratio of CD4＋ to CD8＋T cells of experimental group was markedly lower, respectively （ P〈0.01）, while difference of spleen index between two groups was no significance （ P 〉 0.05）. BALB/C mice xenograft lung cancer models Conclusion The use of with subcutaneous injection CsA would improve success rate of of human lung adenocarcinoma cell line A549.

关 键 词：环孢菌素A 模型 动物 肺肿瘤 免疫 细胞 

分 类 号：R734.2[医药卫生—肿瘤]