壳聚糖-滔罗定纳米凝胶对CO_2气腹后HeLa细胞侵袭性的抑制作用  

Suppressive Effect of Chitosan-taurolidine Nanogel on Invasiveness of HeLa Cells Treated with CO_2 Pneumoperitoneum Pressure

作　　者：杨琰[1] 黄颢[1] 严兆华[1] 吕炎[1] 张凤兰[1] 

机构地区：[1]广东医学院附属医院妇产科,湛江524001

出　　处：《华中科技大学学报（医学版）》2013年第3期342-345,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：广东省医学科学技术研究基金资助项目(No.B2010247);广东医学院博士启动基金资助项目(No.B2009011)

摘　　要：目的探讨纳米载体壳聚糖(chitosan,CS)包裹新型抑癌剂滔罗定(taurolidine,TRO)所合成的纳米凝胶对CO2气腹后HeLa细胞侵袭性的抑制作用。方法以0.02%的壳聚糖为纳米载体,制备滔罗定凝胶,采用扫描电镜观察新鲜制备的纳米凝胶形态。以终浓度为0.2mg/mL的壳聚糖-滔罗定凝胶处理HeLa细胞24h后,Transwell小室法检测细胞处理前后侵袭及迁徙能力的改变。结果所制备的壳聚糖-滔罗定凝胶为匀质、透明的胶样液体,电镜下凝胶为大量纤维交织形成支架的匀质结构,支架间空隙直径为50～300μm。CO2气腹后HeLa细胞侵袭及迁徙的数目为(69.2±1.5)和(90.5±4.1),经壳聚糖-滔罗定凝胶处理后,数目分别减少到(20.8±2.5)和(61.3±2.8),差异有统计学意义(均P<0.05)。结论壳聚糖-滔罗定凝胶能很好抑制CO2气腹后HeLa细胞的侵袭及迁徙特性,有可能成为腹腔镜CO2气腹后减少肿瘤转移的较好的治疗方式。Objective To investigate the suppressive effect of the chitosan-taurolidine nanogel on the invasiveness of HeLa cells treated with CO2 pneumoperitoneum pressure.Methods Chitosan（0.02%）,as a nano carrier,was used to synthesize the taurolidine nanogel.The morphology of the fresh-prepared nanogel was observed by the scanning electron microscopy.The invasive ability of HeLa cells was detected by the Transwell migration assay before and after chitosan-taurolidine nanogel treatment.Results Scanning electron microscopy revealed that the chitosan-taurolidine nanogel presented a homogenous structure with a large quantity of fiber interwoven to form a network.The interspace of the network was 50-300μm in diameter.After treatment with CO2 pneumoperitoneum pressure,the number of invading and migrating HeLa cells was（69.2±1.5）and（90.5 ±4.1）and it was decreased to（20.8±2.5）and（61.3±2.8）after chitosan-taurolidine nanogel treatment with the difference being statistically significant（P〈0.05）.Conclusion The chitosan-taurolidine nanogel could restrain the invasion and migration of HeLa cells treated with CO2pneumoperitoneum pressure,which suggested that it may become a potential treatment alternative that can reduce tumor metastasis after laparoscopic CO2 pneumoperitoneum pressure treatment.

关 键 词：腹腔镜手术 二氧化碳气腹 宫颈癌 壳聚糖 滔罗定 肿瘤侵袭 

分 类 号：R737.33[医药卫生—肿瘤]