咖啡对糖尿病大鼠胰岛素分泌、敏感性及氧化应激影响  

Effect of coffee on insulin secretion, sensitivity and oxidative stress in diabetic rats

作　　者：陈琳[1] 喻明[1] 夏娟[1] 高月锦[1] 

机构地区：[1]上海市普陀区中心医院内分泌科,上海200062

出　　处：《中南药学》2013年第6期424-428,共5页Central South Pharmacy

基　　金：上海市教育委员会预算内项目(编号:2010JW63)

摘　　要：目的评价咖啡对链脲佐菌素(STZ)诱导的糖尿病大鼠胰岛素敏感性、氧化应激指标及胰腺内胰岛素含量的影响。方法 Wistar大鼠48只,随机分为正常对照组(NC组)、含咖啡因咖啡灌胃组(CC)、去咖啡因咖啡灌胃组(DC)、STZ模型组(S)。CC组和DC组分别以咖啡和去咖啡因咖啡每日灌胃,8周后CC组、DC组、S组均腹腔注射STZ 10 mg kg-1,行口服葡萄糖耐量试验(OGTT)及胰岛素耐量试验(ITT),测定血浆8-异前列腺素F2α(8-isoPGF2α)和8-羟脱氧鸟苷(8-OHDG)水平,检测胰腺内胰岛素含量。结果 OGTT CC组、DC组、S组均较NC组血糖明显升高。ITT结果 DC组和CC组ITT 30、60、120 min血糖下降比例明显高于S组、与NC组比较血糖下降比例无显著差异。CC组、DC组、S组血浆8-isoPGF2α和8-OHDG水平较NC组明显升高,CC组和DC组8-isoPGF2α和8-OHDG水平显著低于S组。CC组、DC组、S组胰腺内胰岛素含量较NC组显著降低,CC组胰腺内胰岛素水平明显高于DC组和S组。结论含咖啡因咖啡和去咖啡因咖啡均可增加糖尿病大鼠胰岛素敏感性、改善氧化应激;含咖啡因咖啡可增加内源性胰岛素分泌。本研究结果提示含咖啡因和去咖啡因咖啡都可能通过影响氧化应激参与调节糖代谢,含咖啡因咖啡可改善糖尿病大鼠B细胞分泌功能。Objective To evaluate the effect of coffee on insulin sensitivity,oxidative stress and pancreatic insulin content in diabetic rats induced by STZ.Methods A total of 48 Wistar rats were randomly divided into a normal control group（NC group）,a caffeinated coffee lavage group（CC）,a decaffeinated coffee lavage group（DC）,and an STZ model group（S）.The CC and DC groups were treated with caffeinated coffee and decaffeinated coffee daily lavage for 8 weeks.STZ 10 mg kg-1was injected before the oral glucose tolerance test（OGTT） and insulin tolerance test（ITT）.Plasma 8-iso prostaglandin F2 alpha（8-isoPGF2 alpha） and 8-hydroxyl deoxyguanosine（8-OHDG） were measured,and insulin levels within the pancreas were detected.Results The CC,DC,and S showed higher blood glucose levels compared with the NC group.ITT showed that the DC and CC groups had higher blood glucose rate at 30,60,and 120 min compared with the S group,and the two groups showed no significant differences in the blood glucose rate compared with the NC group.The CC,DC,and S groups had elevated plasma 8-isoPGF2 alpha and 8-OHDG.However,the level in the CC and DC groups was significantly lower than that in the S group.Although the insulin levels in the pancrea were all significantly decreased in the CC,DC,and S groups,the pancreatic insulin content in the CC group was obviously higher than that in the DC and S groups.Conclusion The impaired insulin sensitivity and oxidative stress in diabetic rats might be improved by both caffeinated and decaffeinated coffee.Caffeinated coffee can promote endogenous insulin secretion.Caffeinated and decaffeinated coffee are both likely to be involved in regulating glucose metabolism by influencing oxidative stress,and caffeinated coffee may improve the beta cell function in diabetic rats.

关 键 词：糖尿病 咖啡 氧化应激 胰岛素 链脲佐菌素 敏感性 

分 类 号：R965[医药卫生—药理学]