多效生长因子在结直肠癌组织中的表达及其临床意义  

The Expression and Clinical Significance of Pleiotrophin in Colorectal Cancer Tissues

作　　者：陈南征[1] 孙学军[1] 贺赛[1] 郑见宝[1] 任燕飞[1] 王炜[1] 魏光兵[1] 姚建锋[1] 张立[1] 

机构地区：[1]西安交通大学医学院第一附属医院普外科,陕西西安710061

出　　处：《中国普外基础与临床杂志》2013年第6期615-619,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金项目(项目编号:81172362);陕西省科学技术研究发展计划项目(项目编号:2011-K12-19)~~

摘　　要：目的探讨多效生长因子(pleiotrophin,PTN)mRNA及其蛋白在结直肠癌组织中的表达及其临床意义。方法采用原位杂交和免疫组化染色方法,检测结直肠癌组织和正常结直肠组织中PTN mRNA及其蛋白的表达,并分析其与结直肠癌患者临床病理特征的关系。结果结直肠癌组PTN mRNA及其蛋白的表达阳性率分别为63.9%(53/83)和60.2%(50/83),均高于(P=0.008,P=0.002)正常对照组的40.7%(22/54)和33.3%(18/54)。结直肠癌组织中PTN mRNA及其蛋白的表达与肿瘤分化程度和TNM分期均有关(P<0.05),Ⅲ～Ⅳ期者和低分化者的表达阳性率均较高;而与年龄、性别及肿瘤类型均无关(P>0.05)。结论 PTN mRNA及其蛋白在结直肠癌组织中高表达,其可能参与了结直肠癌的浸润和转移。PTN可作为预测结直肠癌转移和预后的指标之一。Objective To identify the expression of pleiotrophin（PTN）mRNA and protein in the colorectal cancer tissues,and to explore the clinical value of it.Methods The expressions of PTN mRNA and protein in colorectal cancer tissues（colorectal cancer group）as well as normal colorectal tissues（normal control group）were tested by using in-situ hybridization and immunohistochemistry.Results The positive rates of PTN mRNA〔63.9%（53/83）vs.40.7%（22/54）〕and protein〔60.2%（50/83）vs.33.3%（18/54）〕in colorectal cancer group were all significantly higher than those of normal control group（P=0.008,P=0.002）.There were no significant relationship between expressions of PTN mRNA and protein with gender,age,and type of tumor（P〈0.05）,but in tissues of Ⅲ-Ⅳ stage and poor differentiation,the positive rates of PTN mRNA and protein were all higher（P〈0.05）.Conclusions The over expressions of PTN mRNA and protein in colorectal cancer tissues may directly related to the invasion and metastasis.Meanwhile,it can be used as an index to predict metastasis and prognosis of colorectal cancer.

关 键 词：多效生长因子 结直肠癌 组织芯片 原位杂交 免疫组化染色 

分 类 号：R735.3[医药卫生—肿瘤]