Cytotoxicity and genotoxicity of multi-walled carbon nanotubes with human ocular cells  

作　　者：YAN Lu LI GuoXing ZHANG Shu SUN Fei HUANG XiaoJie ZHANG Qian DAI LiMing LU Fan LIU Yong 

机构地区：[1]School of Ophthalmology & Optometry,Eye Hospital,Wenzhou Medical College [2]Institute of Advanced Materials for Nano-Bio Applications,Wenzhou Medical College [3]Center of Advanced Science and Engineering for Carbon (Case4Carbon),Department of Macromolecular Science and Engineering,Case Western Reserve University

出　　处：《Chinese Science Bulletin》2013年第19期2347-2353,共7页

基　　金：supported by the National Natural Science Foundation of China (81000663);Key International Cooperation Project of the Ministry of Science and Technology of China(2009DFB30380);Research Fund for the Doctoral Program of Higher Education of China (20103321120003);Key Project for Science and Technology Research of Ministry of Education of China (211069);the Scientific Research Foundation for the Returned Overseas Chinese Scholars;the Innovation Team of Ministry of Education of China (IRT1077);the National "Thousand Talents Program"

摘　　要：Cytotoxicity and genotoxicity of plasma-modified multi-walled carbon nanotubes (MWCNTs), including hydroxyl-MWCNTs (MWCNT-OH), carboxyl-MWCNTs (MWCNT-COOH) and pristine MWCNTs, with human ocular cells (e.g. retinal pigment epithelium (RPE) cells) have been studied in this work. The addition of MWCNT-based materials caused few change in cell morphology while the presence of MWCNTs was observed inside the cells using transmission electron microscopy (TEM), suggesting possibility of MWCNTs passing through the cell membranes without damaging cells. Cell viability measurements suggested that MWCNT-COOH exhibited better biocompatibility than other MWCNT materials studied in this work. Lactate Dehydrogenase (LDH) release level was found to be less than 30% with all types of MWCNT-based materials. Reactive Oxygen Species (ROS) generation was visible but not severe with addition of nanotubes. A smaller oxidative stress level was obtained from MWCNT-COOH. Cell apoptosis was found to be less than 1.5% with addition of MWCNT-based materials. Particularly MWCNTs were found to be swallowed by cells and released by cells after 72 h without damaging cells, which may be considered as a potential vector for ocular genetic diseases. Plasma modification of MWCNTs particularly with-COOH was found to be an efficient way to improve ocular biocompatibility of MWCNTs, suggesting a fast and useful way to modify MWCNTs for applications in areas such as biology and biomedicine.Cytotoxicity and genotoxicity of plasma-modified multi-walled carbon nanotubes （MWCNTs）, including hydroxyl-MWCNTs （MWCNT-OH）, carboxyl-MWCNTs （MWCNT-COOH） and pristine MWCNTs, with human ocular cells （e.g. retinal pigment epithelium （RPE） cells） have been studied in this work. The addition of MWCNT-based materials caused few change in cell morphology while the presence of MWCNTs was observed inside the cells using transmission electron microscopy （TEM）, suggesting possibility of MWCNTs passing through the cell membranes without damaging cells. Cell viability measurements suggested that MWCNT-COOH exhibited better biocompatibility than other MWCNT materials studied in this work. Lactate Dehydrogenase （LDH） release level was found to be less than 30% with all types of MWCNT-based materials. Reactive Oxygen Species （ROS） generation was visible but not severe with addition of nanotubes. A smaller oxidative stress level was obtained from MWCNT-COOH. Cell apoptosis was found to be less than 1.5% with addition of MWCNT-based materials. Particularly MWCNTs were found to be swallowed by cells and released by cells after 72 h without damaging cells, which may be considered as a potential vector for ocular genetic diseases. Plasma modification of MWCNTs particularly with-COOH was found to be an efficient way to improve ocular biocompatibility of MWCNTs, suggesting a fast and useful way to modify MWCNTs for applications in areas such as biology and biomedicine.

关 键 词：多壁碳纳米管 细胞毒性 遗传毒性 人眼 碳纳米管材料 等离子体改性 透射电子显微镜 生物相容性 

分 类 号：R77[医药卫生—眼科] TB383.1[医药卫生—临床医学]