Hepcidin mRNA在酒精性肝病小鼠肝脏中的表达  

作　　者：施文祥[1] 任源[1] 

机构地区：[1]桂林医学院公共卫生学院,广西541004

出　　处：《当代医学》2013年第19期16-17,共2页Contemporary Medicine

基　　金：广西自治区教育厅资助项目(200103YB090)

摘　　要：目的研究hepcidin mRNA在酒精性肝病(ALD)小鼠肝脏的表达。方法将16只小鼠随机分为对照组和模型组,每组8只。以酒精持续灌胃12周制作ALD模型,对照组等量蒸馏水灌胃。第12周末处死小鼠,观察各组小鼠肝脏病理变化,并测定肝功能。用Real-time PCR方法测定模型组和对照组小鼠肝脏hepeidin mRNA含量。结果与对照组(0.060±0.015)比较,模型组hepcidin mRNA(0.008±0.002)表达明显降低(P<0.01)。结论 hepcidin mRNA在ALD小鼠肝脏的表达明显降低,从而导致肠铁吸收增加,为进一步研究ALD发病机制提供新的思路。Objective To investigate liver hepcidin mRNA content on mice with alcoholic liver disease（ALD）. Methods Sixteen C 57/BL 6 mice were randomLy divided into control group and model group. ALD was induced by gavage of alcohol for 12 weeks. Control group were administered with tales doses distilled water. Liver injuries were assessed by histopathologic examination and serum ALT levels. Liver hepcidin mRNA content was determined by real-time PCR. Results Compared with control group, liver hepcidin mRNA content in model group had significant decreased. Conclusion In mouse liver tissue, expression of the iron-sensor peptide hepcidin has been shown to be affected by chronic alcohol consumption. Hepcidin mRNA expression was significantly decreased in model mice compared to the controls, which results increased iron absorption from the intestine, hepcidin might might provide a new study way on ALD.

关 键 词：酒精性肝病 HEPCIDIN 铁过载 

分 类 号：R575.5[医药卫生—消化系统]