镧、铈对铜胁迫下豌豆幼苗抗氧化酶系统的影响  

Effect of La And Ce on Antioxidant Enzymes System of Pea Seedlings under Copper Stress

作　　者：王蕊[1] 王应军[1] 马星宇[1] 

机构地区：[1]四川农业大学资源与环境学院,四川成都611130

出　　处：《核农学报》2013年第6期873-878,共6页Journal of Nuclear Agricultural Sciences

基　　金：四川省教育厅重点基金项目(10ZA059)

摘　　要：本试验以豌豆为研究对象,研究了豌豆幼苗在受到高浓度Cu离子(100mg.L-1)胁迫下,经2种稀土(La、Ce)元素分别处理后对豌豆幼苗的细胞抗氧化酶活性及脂质过氧化产物MDA含量的影响。结果表明,Cu离子胁迫降低了豌豆幼苗SOD、POD和CAT活性,增加了膜脂过氧化产物MDA含量。适宜浓度的La(5～10mg.L-1)和Ce(5～20mg.L-1)可分别有效提高豌豆幼苗的3种抗氧化酶活性并降低MDA含量,显著减轻了Cu胁迫对豌豆幼苗造成的毒害效应(P<0.05)。这说明La、Ce处理能有效提高细胞清除活性氧的能力,一定程度上缓解了Cu离子胁迫对豌豆幼苗膜系统带来的伤害。进一步研究还表明,La处理后对提高豌豆幼苗抗氧化酶活性和降低MDA含量的能力要显著优于Ce。但高浓度(≥40mg.L-1)的两种稀土反而会加剧Cu对豌豆幼苗抗氧化酶系统的伤害,表现出与Cu离子协同胁迫的毒害效应。In the present study,the impact of two rare earth（La and Ce） treatment to the response of enzymatic protective systems and MDA content variations of pea seedlings under high copper stress（100mg·L-1） were researched.The results showed that the Cu stress decreased the activities of SOD,POD and CAT in pea seedling leaves and increased the content of membrane lipid peroxidation products MDA.Appropriate concentration of Lanthanum（0~10mg·L-1）and Cerium（0~20mg·L-1）could effectively increase the activities of SOD,POD and CAT,meanwhile,decrease the content of MDA and significantly alleviated the toxic effect of Cu stress to pea seedlings（P0.05）.It means that the capability of active oxygen removing could be effectively improved under La、Ce processing,and alleviated the damage of cell membrane system to some degree.Further study also showed that the ability of improving pea seedling antioxidant activity and decreasing the content of MDA La was significantly better than Ce.However,high concentration（≥40mg·L-1）treatment of the two earth could aggravate the toxic effect of Cu on pea seedlings,which showed the stress of collaborative effect with Cu ion.

关 键 词：镧 铈 铜胁迫 豌豆 抗氧化酶 

分 类 号：X173[环境科学与工程—环境科学]