百草枯诱导的慢性帕金森病模型大鼠血脑屏障和P-gp变化的研究  

Study on the alteration of blood-brain barrier function and expression of P-gp in rats with paraquat-induced chronic parkinson disease

作　　者：孟珊珊[1] 贾非 刘洪文[1] 赵庆杰[3] 张杰[1] 

机构地区：[1]哈尔滨医科大学附属第一医院大桥分院,哈尔滨150076 [2]武警牡丹江市森林支队 [3]哈尔滨医科大学附属第一医院神经内科

出　　处：《临床急诊杂志》2013年第6期273-276,共4页Journal of Clinical Emergency

基　　金：黑龙江省教育厅科学技术研究项目(No:12511263)

摘　　要：目的:探讨百草枯诱导的慢性帕金森病模型中大鼠血脑屏障功能和P-糖蛋白表达的变化。方法:随机将动物分成3组:对照组(生理盐水注射组)和模型组(百草枯注射4周、8周组)。以免疫组织化学染色方法显示各组中脑酪氨酸羟化酶(TH)阳性细胞、免疫印迹检测TH蛋白表达和P-糖蛋白表达、Belyaevs'法使用EB测定BBB通透性。进行统计学分析。结果:与对照组相比,模型组TH阳性神经元和TH蛋白表达较对照组明显减少(P<0.05);模型组大鼠BBB的通透性显著增加(P<0.05);与对照组比较,模型组P-gp表达显著降低(P<0.05)。结论:百草枯可通过使TH阳性神经元和TH蛋白表达减少,诱导大鼠慢性帕金森病模型;同时导致BBB通透性增加和P-糖蛋白表达下降,百草枯可能通过影响BBB通透性和P-糖蛋白的表达诱导帕金森病。Objective：To investigate the alteration of blood-brain barrier （BBB） measured by EB and expression of P-gp in paraquat-induced chronic Parkinson disease. Method： Thirty male Wistar rats were randomly divided into three groups： normal group（physiologic saline group） and two model groups （paraquat 4 weeks and 8 weeks group） ,The number of tyrosine hydroxylase （TH）- positive neurons were observed by immunohistochemistry, TH protein and the P-gp expressions were examined by immuno blotting, the BBB permeability was assessed by Belyaevs＇ method. Result： Compared with normal group, TH-positive neurons and the expressions of TH in the model groups were obviously reduced （P〈0.05） ;the BBB permeability of the model groups was obviously deduced compared with normal group （P〈0. 05）, P-gp protein expression in the model groups was downregulated obvious- ly（P〈0.05）. Conclusion：The chronic PD modal were induced by paraquat which leaded to the down-regulation of TH-positive neurons and the expressions of TH. At the same time, paraquat induced the increasion of BBB permea- bility and reduced P-gp protein expression. Paraquat might have certain effect on chronic PD by regulating BBB permeability and P-gp protein expression.

关 键 词：帕金森病 百草枯 血脑屏障 P-糖蛋白 

分 类 号：R742.5[医药卫生—神经病学与精神病学]