二乙基亚硝胺诱发大鼠肝癌肝细胞超微结构及葡萄糖—6—磷酸酶细胞化学的观察  

Observation of Hepatocellular Ultrastructure and G-6-Pase Cytochemistry in Hepatocarcinogenesis Induced by Diethylnitrosamine in Rats

作　　者：颜永碧[1] 夏愿耀[1] 叶煦亭[1] 丛文铭[2] 吴孟超[2] 张秀忠[2] 

机构地区：[1]第二军医大学训练部中心实验室,200433 [2]长海医院肝胆外科研究所,200433

出　　处：《第二军医大学学报》1991年第2期139-142,共4页Academic Journal of Second Military Medical University

摘　　要：二乙基亚硝胺诱发实验性肝癌过程中,用电镜观察肝结节内肝细胞及G-6-Pase的改变,结果显示:(1)肝细胞问的连接较正常为少,部分区域的细胞连接消失,细胞问隙增宽。(2)部分肝细胞核有凹陷,核仁肿大,胞质内线粒体嵴变短而少,粗面内质网膜上核糖体脱落,糖原颗粒减少。(3)少数细胞的细胞核增大,胞质内游离核糖体丰富,各细胞器不发达。(4)诱癌第8周,肝细胞内G-6-Pase呈阳性反应,12周后为阴性。这些特点可认为是肝细胞去分化的主要指示。In the process of hepatocarcinogenesis induced by diethylnitrosamine (DENA) in rats, the hepatocellular ultrastructure and G-6-Pase reactions in hepatic nodes were observed by electron microscope. The results are as follows: As compared with normal hepatocytes, cell junctions were fewer, even disappeared in some areas and intercellular spaces were wider; in some cells, nuclear membranes invaginated into the nucleoplasms frequently, micleoli were enlarged, mitochondria appeared swollen and their cristae were scanty- and short, and depolymerized ribosomes dropped off the dilated rough endoplasmic reticulums; in some seriously diseased cells, nucleoli were enlarged, abundant free ribosomes were present, but the' other organelles were in lower differencial state. G-6-Pase reactions were positive before the 8th week of DENA induction and negative after the 12th week. These suggest the hepatocellular metabolic disturbance and low differenciation.

关 键 词：肝肿瘤 肝细胞 细胞化学 超微结构 

分 类 号：R735.702[医药卫生—肿瘤]