香蕉枯萎病菌2种细胞壁降解酶酶活性平板检测方法  

Plate Assays for Measurement of Two Kinds of Cell Wall Degrading Enzymes Activity in Fusarium oxysporum f. sp. cubense

作　　者：施金秀[1] 林先丰[1] 姜乃月[1] 凌金锋[1] 李敏慧[1] 姜子德[1] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642

出　　处：《华南农业大学学报》2013年第3期340-344,共5页Journal of South China Agricultural University

基　　金：国家自然科学基金(31200112);现代农业产业技术体系建设项目专项(CAR-32-05);华南农业大学校长基金(2007K060)

摘　　要：以病原真菌香蕉枯萎病菌4号生理小种为材料,建立了多聚半乳糖醛酸酶和纤维素酶的平板检测方法.多聚半乳糖醛酸酶的最佳产酶和检测条件为:诱导底物为果胶,接种孢子终浓度为1×104mL-1,培养时间为3 d.37℃时检测的多聚半乳糖醛酸酶活性最大;纤维素酶的最佳产酶和检测条件为:诱导底物为羧甲基纤维素,接种孢子终浓度为1×105mL-1,培养时间为3 d,37℃时检测的纤维素酶活性最大.在此基础上,筛选已获得的香蕉枯萎病菌4号生理小种致病力减弱突变体,获得了2株多聚半乳糖醛酸酶和纤维素酶酶活性显著降低的致病突变体.This paper reports a plate assay to detect activities of polygalacturonase and cellulase secreted by Fusarium oxysporum f.sp.cubense（FOC）,which is the most destructive pathogen of banana.The optimum cultural conditions for the polygalacturonase production were using pectin as induced substrate to inoculate with 1 × 104 mL-1 conidia of FOC and after being cultured for 3 days.The optimal temperature of polygalacturonase activity was 37 ℃.The optimum cultural conditions for cellulase production were using carboxymethyl cellulose（CMC） as induced substrate to inoculate with 1 × 105 mL-1 conidia of FOC in inducing medium after being cultured for 3 days.The optimal temperature of cellulase activity was 37 ℃.Based on the method,two mutants with impaired polygalacturonase and cellulose activity were attained through screening virulence-reduced mutants of FOC.

关 键 词：香蕉枯萎病菌 纤维素酶 多聚半乳糖醛酸酶 酶活性平板检测 致病突变体 

分 类 号：S432.1[农业科学—植物病理学]