奶牛隐性乳房炎病原菌的分离鉴定与DGGE追溯  

Isolation and Identification of the Cow Subclinical Mastitis Pathogens and DGGE Retrospective Analysis

作　　者：高潮[1,2] 刘国庆[1] 连英琪[1] 朱明[1] 余晓峰[3] 张克春 

机构地区：[1]合肥工业大学,合肥230009 [2]上海市奶牛研究所,上海200436 [3]安徽省出入境检验检疫局,合肥230022

出　　处：《上海交通大学学报（农业科学版）》2013年第3期88-94,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

摘　　要：通过对隐性乳房炎病原菌的分离鉴定和药敏试验,以及对治疗前的奶样进行DGGE追溯性分析,研究乳房炎奶样中微生物区系结构对乳房炎的治疗和复发的影响。结果显示,引起奶牛隐性乳房炎的主要病原菌为大肠杆菌、金黄色葡萄球菌、无乳链球菌、表皮葡萄球菌以及克雷伯菌。通过对大肠杆菌性乳房炎奶样进行DGGE追溯性分析,发现乳房炎奶样中除大肠杆菌外,还有假单胞菌、芽孢杆菌、链球菌、沙雷氏菌等其他的致病菌,并且奶样中微生物区系结构与奶牛乳房炎的治愈时间和乳房炎复发有着紧密的联系,区系结构越复杂,治愈的时间越长,越容易复发,引起后期乳房炎复发的病原菌包含在治疗前的奶样中。In order to clarify the impacts of microbial community structure in mastitis milk samples upon the treatment and recurrence of mastitis, in this study, we performed isolation, identification and susceptibility test of pathogenic bacteria of recessive mastitis and retrospective analysis of DGGE in milk samples before the treatment of mastitis. The results showed that the major pathogenic bacteria causing recessive mastitis in diary cattle were Escherichia coli , Staphylococcus aureus , Streptococcus agalactiae , Staphylococcus epidermidis and Klebsiella. Based on the retrospective analysis of DGGE in milk samples of E. coli mastitis, it was found that Pseudornonas, Bacillus, Streptococcus, Serratia and other pathogenic bacteria were detected in the milk samples except E. coll. In addition,the microbial community structure in milk samples indicated close relationship with the healing time and recurrence of cow mastitis. The more complicated the community structure, the more time it should cost for the mastitis to heal and the more opportunities there are for mastitis to recur.

关 键 词：牛奶 乳房炎 细菌 DGGE 

分 类 号：S858.23[农业科学—临床兽医学]