籽瓜多糖的提取分离及单糖组成的GC-MS分析  

Extraction and separation of polysaccharides from uatermelon seeds and its monosaccharide composition analysis by GC-MS

作　　者：宋珅[1] 赵保堂[1] 殷振雄[1] 刘琴[1] 梁俊玉[1,2] 张继[1,2] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]甘肃特色植物有效成分制品工程技术研究中心,甘肃兰州730070

出　　处：《食品与发酵工业》2013年第5期238-245,共8页Food and Fermentation Industries

基　　金：枸杞等西北特产资源生态高值利用技术研究与产品开发(国家科技支撑计划)(2012BAD36B04)

摘　　要：用微波辅助萃取技术从籽瓜中提取籽瓜多糖,并用GC-MS分析其单糖组成。依据响应面设计(response surface methodology,RSM)原理,使用SAS(SAS Package)软件的中心组合设计建立提取数学模型,对影响籽瓜多糖得率的提取时间、微波功率、提取温度和料液比4个因素进行优化组合。确定最优提取工艺参数为时间19.5min、温度62℃、料液比(g∶mL)1∶0.9、功率302 W。多糖得率为4.58%。对得到的籽瓜多糖进行了红外扫描,3 408、2928、1 408、1 645.08、1 074 cm-1都出现了糖类的特征吸收峰。GC-MS分析结果表明籽瓜多糖由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖六种单糖组成,摩尔比为4.48∶13.14∶4.16∶7.82∶10.1∶60.3。Microwave-assisted extraction（MAE） of polysaccharides from watermelon seeds and its monosaccharide composition by GC-MS was studied.The four parameters,extraction time,microwave power,extraction temperature and the ratio of solid to water were optimized using the Box-Behnken design（BBD） and the quadratic regression model was built by response surface methodology（RSM）.The optimal extraction conditions for were as follows： extraction time19.5 min,extraction temperature 62℃,ratio of raw material to liquid 1∶ 0.9,microwave power 302W.Under the above conditions,the highest yield was 4.58%.The polysaccharides were characterized by FT-IR.The peaks appeared at 3 408 cm-1,2 928 cm-1,1 645 cm-1,1 408 cm-1,1 074 cm-1.The result of GC-MS showed that polysaccharides was mainly composed of six monosacharides： rhamnose,arabinose,xylose,mannose,glucose,and galactose and the molar ratio was 4.48∶ 13.14∶ 4.16∶ 7.82∶ 10.1∶ 60.3 respectively.

关 键 词：籽瓜多糖 微波提取 响应面法 GC-MS分析 

分 类 号：TQ914[化学工程] O657.63[理学—分析化学]