尾静脉液压法导入人白细胞介素-10质粒在大鼠牙周膜的表达  

In vitro expression of human interleukin-10 in periodontal ligament of rats—using hydrodynamic tail vein injection

作　　者：郭建斌[1] 闫福华[2] 马守治[3] 陈江[1] 

机构地区：[1]福建医科大学附属口腔医院种植科,福州350002 [2]南京大学口腔医学院南京市口腔医院牙周科,210008 [3]福建医科大学口腔医学院基础部,福州350002

出　　处：《转化医学研究（电子版）》2013年第1期1-12,共12页Translational Medicine Research(Electronic Edition)

基　　金：国家自然科学基金(30973326,30840092);福建省科技重点资助国际合作项目(2010I0006)的资助~~

摘　　要：目的探讨尾静脉液压法导入质粒后外源基因在大鼠牙周膜的表达情况。方法选用24只SD大鼠，随机分为h1L-10组与Veetor组，尾静脉液压法导入质粒后，肝脏冰冻切片及血清检查外源基因的表达。取上颌第二磨页牙周膜，观察免疫组织化学法h1L-10的表达情况。结果荧光镜下可见她肝脏冰冻切片显示人丛绿色荧光蛋白的表达；采用尾静脉液压法导入h1L-10质粒24h后肝脏组织巾有较离的h1L-10蛋h表达。导人h1L-10质粒4h后即检测到h1L-10表达，8h时可达高峰。牙周膜区域未显示明显的h1L-10蛋白表达。未能检测到空载体组大鼠h1L-10蛋白的表达，结论尾静脉液压法导入h1L-10质粒后能使外源基因在大鼠体内获得相对稳定的表达，但在大鼠牙刷膜区域未能观察到h1L-10蛋白的表达。Objective To investigate the expression of Human Interleukin-10（IL-10） gene in the periodontal ligament of rats via hydrodynamic tail vein injection. Methods Twenty four Sprague I）awley @ （SD） rats were randomly divided into hlL-l0 group and the vector gnoup. After plasmid was introduced into rats via hydrodynamic tail vein injection, frozen liver sections and serum were used to detect the exogenous Human Interleukin-l0gene expression. The periodontal ligament at furcation area of maxillary second molar was taken for examination of hIL-10 expression by immunohistochemistry. Result Fro- zen liver sections showed a large number of bright green fluorescent proteins, and hIL-10 proteins were highly expressed in the liver 24 h after injection. The expression ofhIL-10 protein could be detected in serum and peaked at 8 h after injection and no obvious h lL-l0 expression was found in periodontal ligament in hIL-10 group. No expression of hlL-10 was detected in vector gvoup. Conclusion A relatively stable expression of exogenous gene in liver can be obtained via hydrodynamic tail vein injec- tion in rats; however, expression of hIL-10 protein was not found in the periodontal ligament.

关 键 词：人白细胞介素-10 尾静脉液压法 牙周膜 质粒 

分 类 号：R781.4[医药卫生—口腔医学]