烟草原生质体的制备和分析  

Preparation and Analysis of Tobacco Protoplasts

作　　者：尚飞[1] 李咪咪[1] 李莉[1] 梁卫红[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2013年第3期130-132,137,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31171182);河南省科技创新杰出青年计划(104100510012);河南省基础与前沿技术研究项目(132300410137);河南省自然科学研究计划项目(2010A180012)

摘　　要：植物原生质体作为一个良好的实验系统,广泛应用于植物分子及细胞学研究中.本研究以烟草NC89叶片为材料,采用酶解法制备烟草原生质体,经台盼蓝染色鉴定活性,结果显示,分离纯化后的原生质体产量为(12.7±1.6)×106 g-1,表明该方法能成功地制备高产率、高活力的原生质体,建立了一种烟草原生质体的简易制备方法.Abstact：Plant protoplast provides us a unique single cell system in biology study.To explore an effective method of protoplast preparation,we take tobacco leaves as material and digest it in enzyme solution,finally use trypan blue staining to identify the activities of protoplast.The results showed that the yield of protoplasts were up to（12.7±1.6）×106/g,higher than most of the recent reports.It suggest that a method had been established for high-yield and high-activity protoplast preparation in this research which has a certain reference value.

关 键 词：烟草 原生质体 分离纯化 酶解法 

分 类 号：Q942[生物学—植物学]