“神州八号”航天搭载诱变选育果胶酶高产菌株  

Breeding of high pectinase-producing strain by “Shenzhou Ⅷ”craft-boarding treatment

作　　者：马成[1] 马伟超[2] 安建平[1,2] 刘西斌[2] 贵强[2] 李师翁[1] 

机构地区：[1]兰州交通大学化学与生物工程学院,甘肃兰州730070 [2]天水师范学院生命科学与化学学院,甘肃天水743001

出　　处：《食品工业科技》2013年第13期184-186,201,共4页Science and Technology of Food Industry

基　　金：甘肃省科技厅资助项目(090NKCE081)

摘　　要：以泡盛曲霉40499为出发菌株,通过"神州八号"航天搭载进行太空诱变选育,共培养得到168株生长菌,经碘液染色法初筛,液态发酵法复筛,得到5株果胶酶高产菌株,分别命名为HP26-2、HP37-5、HP39-1、HP45-1、HP48-3,其中HP26-2酶活最大,达到17.162U/mL,比对照菌株高1.26倍,HP37-5和HP39-1在发酵时间60～132h持续产酶能力最强,产酶能力最稳定。The strain Aspergillus awamori 40499 was aboard in ＂Shenzhou VIII＂ spacecraft to carry out space mutation,and 168 mutant strains were isolated.Among them,5 high pectinase-producing strains were obtained after preliminary screening using 12 staining method ,and re-screening by liquid fermentation test,they were named HP26-2;HP37-5 ;HP39-1 ;HP45-1 ;HP48-3, respectively.The activity of pectinase produced by HP26-2 was the highest,reached 17.162U/mL, which was 1.26 times higher than that produced by wild strain. The pectinase continuous- producing ability of HP37-5 and HP39- 1 were stronger and more stable, they were producing pectinase steadily in the period of 60-132h fermentation.

关 键 词：泡盛曲霉 航天诱变 果胶酶活 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]