HPLC同时测定桑葚口服液中槲皮素和山奈酚的含量  

Determination of Quercetin and Kaempferol in Mulberry Oral Liquid by HPLC

作　　者：张媛[1] 李立[2] 王晓杨[1] 张志琴[1] 

机构地区：[1]金华职业技术学院医学院,金华321016 [2]江西中医学院,南昌330006

出　　处：《江西中医学院学报》2013年第1期46-48,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：浙江金华市科技局项目;金华市自然科技基金项目(项目编号:2007-3-055)

摘　　要：目的:建立桑葚口服液中槲皮素、山奈酚成分的HPLC测定。方法:采用HPLC法Symmetry C18柱(150mm×4.6mm5μm);流动相:甲醇-0.4%磷酸(60 40);检测波长:370nm;流速为0.5mL min-1。对样品水解过程的各个影响因素进行了考察。结果:优化的水解条件为采用2.0mol L-1盐酸甲醇溶液,90℃水浴水解2.0h。槲皮素和山奈酚分别在峰面积与浓度呈良好的线性关系,范围分别为0.18-35.28mg L-1,0.19-38.22mg L-1,线性关系良好(r=0.999 8)。槲皮素加样回收率为99.43%,RSD为1.16%(n=6);山柰酚加样回收率为99.19%,RSD为2.04%(n=6)。结论:该法操作简便、结果可靠,重现性好,可用于控制桑葚口服液的质量。Objective： To develop a high performance liquid chromatographic method for the determination of quercetin and kaempferol in mulberry oral liquid Method： The optimized method was achieved for the separation and detection of quercetin and kaempferol us ing Symmetry C18 column（150mm×4.6mm 5μm） as the stationary phase,methanol-0.4% aqueous phosphoric acid solution（60 40） as the mobile phase at a flow rate of 0.5 mL min-1,370 nm as the detection wavelength.The main,which influenced the Sample hydro lysis procedure,were intensively explored.Result： The optimized hydrolysis procedure was that the sample was hydrolyzed 2.0 h with 2.0 mol L-1 hydrochloric acid methanol at 90 ℃.Quercetin and kaempferol showed good relationship at the range of 0.18-35.28 mg L-1,0.19-38.22mg L-1,respectively.Both of the correlation coefficients of the calibration curves were 0.999 8.The average recoveries of quercetin and kaempferol were 99.43% and 99.19% with relative standard derivation（RSD） of 1.16% and 2.04%.Conclusion： The re sults showed that the method is simple,accurate and repeatable and it is suitable for quality control of mulberry oral liquid.

关 键 词：槲皮素 山奈酚 HPLC 桑葚口服液 

分 类 号：R284.1[医药卫生—中药学]