载BRL细胞聚鸟氨酸微胶囊的制备与功能  

Preparation and function of BRL-encapsulated poly-L-ornithine microcapsule

作　　者：刘源岗[1] 白燕[1] 王士斌[1] 

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《功能材料》2013年第12期1804-1807,共4页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(31000441;31170939);中央高校基本科研业务费资助项目(JB-JC1009;JB-SJ1009);福建省高等学校杰出青年科研人才培育计划资助项目(JA12004);福建省自然科学基金资助项目(2011J01223)

摘　　要：为了研究新型膜材聚鸟氨酸对微囊化细胞功能的影响,制备了包埋有BRL细胞的聚鸟氨酸-海藻酸盐微胶囊并考察了微囊化肝细胞的生物学活性以及细胞功能。结果表明,载BRL细胞的PLO微胶囊形态完整,表面光滑,细胞在PLO微胶囊内的三维立体环境中能够维持活性,且具有维持分泌尿素和蛋白质的功能,从而为该新型膜材在细胞微囊化的应用方面提供了实验依据。To investigate the influence of poly-L-ornithine on the functions of microencapsulated cells, in this ar- ticle, BRL-encapsulated poly-L-ornithine/alginate microcapsules were prepared, and the cell viability and the cell functions were studied. The results showed that the BRL-encapsulated poly-L-ornithine microcapsules had excellent integrity and smooth surface. The BRL cells maintained cell viability and the functions to secrete urea and protein in the 3D microenvironment of poly-L ornithine microcapsules, and it would supply experimental data in the future application of poly L ornithine as new membrane material in the filed of cell microencapsula- tion.

关 键 词：聚鸟氨酸 BRL细胞系 微胶囊 细胞功能 

分 类 号：R318[医药卫生—生物医学工程]