七氟醚对顺铂和γ射线抑制人肺腺癌A549细胞生长作用的影响  

Effects of sevoflurane on inhibition of growth of human lung adenocarcinoma A549 cells by cisplatin and γ ray

作　　者：梁桦[1] 廖美娟[1] 赖晓红[1] 黄翔[1] 杨承祥[1] 

机构地区：[1]中山大学附属佛山医院麻醉科,佛山市528000

出　　处：《中华麻醉学杂志》2013年第4期455-458,共4页Chinese Journal of Anesthesiology

基　　金：广东省佛山市卫生局医学科研课题（2013030）

摘　　要：目的探讨七氟醚对顺铂和γ射线抑制人肺腺癌A549细胞生长作用的影响。方法人肺腺癌A549细胞接种于培养板，培养24h后，采用随机数字表法，将其分为6组（n=6）：对照组（C组）、七氟醚组（s组）、顺铂组（D组）、顺铂＋七氟醚组（DS组）、γ射线组（R组）和γ射线＋七氟醚组（Rs组）。C组不接受药物处理；S组用2．5％七氟醚孵育4h；D组加入终浓度为3mg／L顺铂孵育4h；Ds组加入终浓度为3mg／L顺铂，并用2．5％七氟醚孵育4h；R组采用γ射线2Gy辐照4h；RS组采用γ射线2Gv辐照，并用2．5％七氟醚孵育4h。每组细胞处理完毕后，继续培养24h，检测克隆形成情况，计算克隆形成率；采用平板克隆法和MTT法检测细胞增殖情况，计算增殖抑制率；采用流式细胞仪检测细胞凋亡率；采用Westernblot法检测x连锁凋亡抑制蛋白（XIAP）和caspase-3的表达水平。结果与c组比较，s组、D组、Ds组、R组和Rs组细胞克隆形成率降低，增殖抑制率和凋亡率升高，XIAP表达下调，easpase-3表达上调（P〈0．05）；与s组和D组比较，Ds组细胞克隆形成降低，增殖抑制率和凋亡率升高，XIAP表达下调，easpase-3表达上调（P〈0．05）；与s组和R组比较，Rs组细胞克隆形成降低，增殖抑制率和凋亡率升高，XIAP表达下调，caspase-3表达上调（P〈0．05）。结论七氟醚可增强顺铂和γ射线抑制人肺腺癌A549细胞生长的作用，其机制可能与下调XIAP表达，上调caspase-3表达有关。Objective To investigate the effects of sevoflurane on inhibition of growth of human lung ade- noearcinoma A549 cells by cisplatin and γ ray. Methods The human lung adenocarcinoma cell line A549 was seeded in culture plate. After being cultured for 24 h, the cells were randomly divided into 6 groups （ n = 6 each） ： control group （group C）, sevoflurane group （group S）, cisplatin group （group D）, cisplatin ＋ sevoflu- rane group （group DS）, γ ray group （group R） and γ ray ＋ sevoflurane group （group RS） . A549 cells were ex- posed to 2.5% sevoflurane for 4 h in group S. Cisplatin with the final concentration of 3 mg/L was added to the culture medium and the cells were then incubated for 4 h in group D. Cisplatin with the final concentration of 3 mg/L was added to the culture medium and the cells were then exposed to 2.5% sevoflurane for 4 h in group DS. A549 cells were exposed to γ irradiation （2 Gy） for 4 h in group R. A549 cells were exposed to y irradiation （2 Gy） and to 2.5% sevoflurane for 4 h in group RS. The cells were cultured for another 24 h after the end of treat- ment, the colony formation was detected and the rate of colony formation was calculated by colony formation assay. Proliferation of A549 cells was measured by plate colony formation and MTF assay and the rate of proliferation inhi- bition was calculated. Cell apoptosis was detected with flow cytometer. The expression of X-linked inhibitor of ap- optosis protein （XIAP） and caspase-3 was detected by Western blot. Results Compared with group C, the rate ofcolony formation was significantly decreased, the rate of proliferation inhibition and percentage of apoptotic cells were increased, XIAP expression was down-regulated and caspase-3 expression was up-regulated in groups S, D, DS, R and RS （ P 〈 0.05）. The rate of colony formation was significantly lower, the rate of proliferation inhibition and percentage of apoptotic cells were higher, XIAP expression was lower and caspase-3 expression was hi

关 键 词：麻醉药 吸入 顺铂 Γ射线 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]