副鸡禽杆菌A、B、C 3个血清型菌株外膜蛋白的差异分析  

Variance Analysis of Outer Membrane Protein of Three Reference Strains of Avibacterium paragallinarum Serotype A,B and C

作　　者：姚东璧[1,2,3] 房慧伶[1] 孙惠玲[2,3] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]北京市农林科学院畜牧兽医研究所,北京100097 [3]畜禽疫病防控技术北京市重点实验室,北京100097

出　　处：《中国畜牧兽医》2013年第6期60-64,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30871867)

摘　　要：为了探索不同血清型副鸡禽杆菌菌株外膜蛋白的差异,本试验采用FOCUSTM Global Fractionation试剂盒制备副鸡禽杆菌A(221株)、B(0222株)、C(Modesto株)3个血清型参考菌株的外膜蛋白,进行SDS-PAGE凝胶电泳。结果表明,不同血清型参考菌株的电泳图谱相似,经凝胶定量软件分析,结果显示,主要条带均有7个,其分子质量相似,分别占A、B和C蛋白质总量的70.85%、68.6%和65.71%。此外共有3个蛋白质条带分子质量相似,但蛋白质含量差异显著(P<0.05)。本试验为副鸡禽杆菌外膜蛋白的进一步研究提供了理论依据。In order to explore the difference of outer membrane protein （OMP） of different serotype of Avibacterium para- gallinarum, the OMP of three reference strains of Avibacteriurn paragallinarurn serotype A （221） ,B（0222） and C （Modesto） were prepared by FOCUSTM Global Fractionation Kit and examined through SDS-PAGE. As a result, three different serotypes of Avibacteriurn paragallinarurn showed similar SDS-PAGE profile. In each samples, there were seven main strips, each had the similar molecular weight which was analyzed by the gel quantitative software. The percentage of serotype A, B and C was 70.85%, 68. 6G and 65. 71~/o, respectively. In addition, the molecular weights of three bands were somewhat identical, whereas the protein content of them were significantly different （P（0.05）. This study provided a theoretical basis for the fur- ther study of the OMP of Avibacterium paragallinarurn.

关 键 词：副鸡禽杆菌 外膜蛋白 SDS-PAGE 凝胶定量 

分 类 号：Q78[生物学—分子生物学]