1,3-丙二醇氧化还原酶及其工程菌研究进展  

Advances on the 1,3-propanediol dehydrogenase and its engineering bacteria

作　　者：王飞[1] 徐浪[1] 杨泽茜[1] 赵博凯[1] 邓文颖[1] 林静[1] 王旭[1] 齐向辉[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《河南科技学院学报（自然科学版）》2013年第3期19-23,共5页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：国家自然科学基金项目(21006041);江苏大学科研立项及百项本科创新项目(11A344;Y11A169;2012JSSPITP1238;08JDG009)

摘　　要：作为合成许多缩聚物单体的1,3-丙二醇(1,3-propanediol,1,3-PD)是本世纪具有广阔市场潜力的化工原料,在化工、医药、食品等领域具有广泛的应用.介绍了1,3-丙二醇氧化还原酶(1,3-propanediol dehydrogenase,PDOR,EC 1.1.1.202)在1,3-PD生产菌代谢途径中的作用,着重综述了PDOR的基因克隆表达情况及其性质特点,分析了PDOR的晶体结构,同时对1,3-PD生物法生产中工程菌的研究进展进行了详细的介绍,并展望了生物法生产1,3-PD的前景,该文有助于加深对PDOR及其工程菌的了解,使其得到更多研究者的重视.As a monomer for polycondensations to produce polyesters,polyethers and polyurethanes,1,3-propanediol is an essential chemical material with a prosperous market potential in this century and has been widely used in the fields of chemical industry,medicine,food and so on.The role of 1,3-propanediol dehydrogenase in the metabolic pathway of 1,3-PD production strains was introduced in this paper.Then,molecular cloning,expression,properties and crystal structure of PDOR were emphatically reviewed,and the engineering bacteria of 1,3-PD was introduced in detail.At last,the microbial production of 1,3-PD was prospected.This study is helpful to understand PDOR and engineering bacteria so that it can make more researchers’ attention to its application.

关 键 词：1 3-丙二醇 1 3-丙二醇氧化还原酶 克隆表达 晶体结构 工程菌 

分 类 号：Q814[生物学—生物工程]