高效液相色谱法测定FOX-12的纯度  

Determination of the Purity of FOX-12 by HPLC

作　　者：马玲[1] 周诚[1] 张毅 王伯周[1] 邢颖[1] 葛忠学[1] 

机构地区：[1]西安近代化学研究所,陕西西安710065 [2]边防学院,陕西西安710108

出　　处：《火炸药学报》2013年第3期31-34,共4页Chinese Journal of Explosives & Propellants

基　　金：总装预研项目(00401010202)资助

摘　　要：通过优化色谱条件,建立了FOX-12纯度的高效液相色谱分析方法。采用C18反相色谱柱(5μm,250mm×4.6mm)分离样品,柱温为50℃,二元流动相乙腈-水体积比为60∶40,流速为0.8mL/min。结果表明,检测波长为280nm,在0.000 2～0.002g/mL范围内线性良好,线性相关系数为0.999 6,该法标准偏差为0.16,变异系数为0.68%,平均回收率为94.78%～97.53%,方法灵敏、准确,可用于FOX-12产品纯度分析。A high performance liquid chromatography （HPLC） method was established for determination of the purity of FOX-12 by optimizing the chromatographic conditions. The sample was separated by C18 column （5μm, 250mm×4.6mm） using binary mobile phase, acetonitrile and water （volume ratio of 60 ： 40） with flow rate of 0.8mL/min at column temperature of 50℃. The results show that the UV detection wavelength is set at 280nm. A good linearity with correlation coefficient of 0. 9996 is obtained in the range of 0. 0002--0. 002 g/mL . The standard deviation of the method is 0.16, the coefficient of variation is 0.68%, and the average recoveries of the method are in the range of 94.78%- 97.53%, indicating that the method is sensitive and accurate, and can he applied in the determination of the purity of FOX-12.

关 键 词：分析化学 高效液相色谱 纯度分析 FOX-12 

分 类 号：TJ55[兵器科学与技术—军事化学与烟火技术] O657.7[理学—分析化学]