大蓟提取物对植物病原真菌的抑菌活性成分研究  

Anti-Phytopathogenic Fungi Active Constituent in Extract from Cirsium japonicum DC.

作　　者：魏朝霞[1] 姚茹瑜[1] 唐嘉义[1] 

机构地区：[1]r云南农业大学生物多样性研究与应用技术国家工程中心,昆明650201

出　　处：《农药》2013年第7期529-532,共4页Agrochemicals

基　　金：国家科技部“十一五”科技支撑计划项目(2007BAD58B01)

摘　　要：[目的]大蓟有广泛的生物活性,具有生物农药的开发潜力。确定其中抗植物病原菌的主要活性成分,以期为其在农药领域的开发利用提供依据。[方法]通过溶剂提取法提取,用生长速率法测定提取物对5种植物病原真菌的抑菌活性,并用柱层析法分离活性物质。[结果]在质量浓度50 g/L时石油醚提取物对石榴枯萎病菌的抑菌活性最高,抑制率达100%,EC50值为5.1 g/L;从中分离出一个三萜类单体化合物,经鉴定为3β,21β-dihydroxyl-20(30)-en-taraxastane。[结论]大蓟对植物病原真菌有良好的抑菌活性,所分离到的单体化合物为大蓟中的主要抑菌活性成分。[Aims] Cirsium japonicum DC.has a wide-spectrum biological activity and possess exploitation potential to be biopesticide.The research aims to explore the main antifungal component to provide basis for the developent and utilization in pesticide field.[Methods] Solvent extraction method was used to extract active components.A growth rate method was used for determining the antifungal activity of the extraction against 5 kinds of pathogens,and active material was separated by Column Chromatography.[Results] At 50 g/L,petroleum ether extract has the best inhibitory effect on Ceratocystis fimbriata with inhibition rate of 100% and with EC50 value of 5.1 g/L.A triterpene monomer compound was separated and identified as 3β,21β-dihydroxyl-20（30）-en-taraxastane.[Conclusions] Daji has an excellent antifungal activity on plant pathogenic fungi,and the monomer compound is the main antifungal component in Daji.

关 键 词：大蓟 植物提取物 抑菌活性 半数有效浓度 活性成分 

分 类 号：TQ450.1[化学工程—农药化工]