不同产地叶下珠药材中总酚含量测定  

Determination of Total Phenols from Phyllanthus urinaria in Different Locations

作　　者：王坤凤[1] 林琛[1] 吴玲芳[1] 李淑青[1] 张兰珍[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2013年第13期110-112,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30171197);北京市自然科学基金项目(7073093);北京中医药大不创新团队项目(2011-CXTD-12)

摘　　要：目的:建立不同产地叶下珠药材中总酚的含量测定方法。方法:以柯里拉京为对照品,三氯化铁-铁氰化钾为显色剂,检测波长744 nm,采用UV测定11个不同产地叶下珠药材中总酚的含量。结果:柯里拉京在0.604～3.02 mg.L-1线性关系良好(r=0.999 1),平均加样回收率99.79%,RSD 2.72%。不同产地叶下珠药材中总酚质量分数5.00%～12.16%。结论:该方法简单准确、重复性好,可用于叶下珠药材中总酚含量的测定。Objective： To establish a method for determination of total phenols from Phyllanthus urinaria in different locations.Method： Total phenols was determined by UV-Vis spectrophotometer with corilagin as reference substance and FeCl3-K3 [Fe（CN） 6]as chromogenic agent,the detection wavelength was at 744 nm.Result： A good linear relationship of corilagin was achieved in the range of 0.604-3.02 mg.L-1（r =0.999 1）,the average recovery was 99.79% with RSD of 2.72%（n = 6）.The content of total phenols from P.urinaria in different locations was different from 5.00% to 12.16%.Conclusion： This established method was simple,accurate and stable which was suitable for determination the content of total phenols from P.urinaria.

关 键 词：叶下珠 总酚 含量测定 不同产地 

分 类 号：R283.6[医药卫生—中药学]