观察乌司他丁在大鼠脑缺血再灌注损伤中的作用  

Observe the effect of Ulinastatin on cerebral ischemia-reperfusion in rats

作　　者：李辉[1] 刘铁成[1] 刘萍[1] 

机构地区：[1]大连市友谊医院,辽宁大连116000

出　　处：《中风与神经疾病杂志》2013年第6期535-537,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的通过对大鼠脑组织及血清中IL-8、ICAM-1浓度的测定,观察乌司他丁在脑缺血再灌注损伤中的作用。方法将健康雄性SD大鼠随机分为对照组(5只)、乌司他丁治疗组(25只,线栓3h后给药)、未用药组(25只)、结扎组(结扎右侧颈总动脉,25只),应用ELISA法检测对照组及后3组在4h(各5只)、12h(各5只)、24h(各5只)、48h(各5只)、72h(各5只)大鼠脑组织及血清中IL-8、ICAM-1的含量。结果结扎组与空白组IL-8及ICAM-1的含量无统计学意义(P>0.05);未用药组与结扎组相对应的时间点数据有明显差异,具有统计学意义(P<0.05);乌司他丁治疗组与未用药组相应时间点数据具有明显差异(除72h外),具有统计学意义(P<0.05)。结论乌司他丁能减轻大鼠脑缺血后的再灌注损伤、机制与抑制炎症反应有关。Objective To observe the effect of ulinastatin in the expression of IL-8、ICAM-1 in serum after cerebral ischemia-reperfusion in rats.Methods All masculine SD rats were randomly assigned to the following groups：control group（5 rats）;treatment group（25 rats）;control group（25 rats）;ligation group（ligature the common carotid artery and the internal carotid artery,25 rats）.ELISA was used to measure the concentration of the IL-8、ICAM-1 in serum of the control group（5 rats） and the other thress groups at five different time points：4 hours（5 rats）、12hours（5 rats）、24 hours（5 rats）、48 hours（5 rats） and 72 hours（5 rats）.Results There was no statistical significance between ligation group and control group,but statistic of all timings between control group and ligation group had statistical significance（P0.05）.Statistical significance was also existed between the treatment group（4h,12h,24h,48h）and control group（P0.05）.Conclusion Ulinastatin could reduce ischemia-reperfusion injuries,the mechanism may be related to inhibit inflammatory reaction.

关 键 词：缺血再灌注损伤 脑保护 炎症因子 乌司他丁 

分 类 号：R743.3[医药卫生—神经病学与精神病学]