太白银莲花皂甙-1诱导胶质瘤U87MG细胞凋亡及机制研究  

Apoptosis of glioma U87MG cells induced by Anemone taipaiensis induced Saponin-1 and its mechanism

作　　者：李娟[1] 程光[2] 申广浩[1] 汤海峰[3] 张赟[4] 费舟[2] 章翔[1] 罗二平[2] 王啸洋[3] 罗鹏[1] 高振辉[1] 

机构地区：[1]第四军医大学生物医学工程系,陕西西安710032 [2]第四军医大学西京医院全军神经外科研究所,陕西西安710032 [3]第四军医大学西京医院药剂科,陕西西安710032 [4]第四军医大学基础部免疫教研室,陕西西安710032

出　　处：《中国现代医学杂志》2013年第11期1-6,共6页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30873402);陕西省自然科学基金(No:2012JM4010)

摘　　要：目的研究太白银莲花皂甙-1对人脑胶质母细胞瘤U87MG凋亡的影响,并探讨其可能的分子机制。方法采用四甲基偶氮唑蓝(MTT)比色法检测不同浓度不同时间(6、24、48、72 h)太白银莲花皂甙-1对U87MG细胞生长增殖的抑制作用;应用倒置显微镜和Hoehst荧光染色、末端脱氧核苷酸转移酶介导的生物素脱氧尿嘧啶核苷酸缺口末端标记法(TUNEL)、膜联蛋白V-异硫氰酸荧光素/碘化丙啶(AnnexinV-FITC/PI)检测太白银莲花皂甙-1作用U87MG细胞后形态的变化和凋亡情况;通过免疫印迹法法检测太白银莲花皂甙-1处理后细胞凋亡相关蛋白Survivin及活化的Caspase-3的表达。结果太白银莲花皂甙-1可明显抑制U87MG细胞的增殖,诱导其凋亡;同时下调Survivin表达,激活了凋亡蛋白Activated Caspase-3的表达。结论体外实验显示,太白银莲花皂甙-1对U87MG细胞具有显著的增殖抑制作用,并能成功诱导人脑胶质母细胞瘤U87MG细胞发生凋亡,其机制可能与抑制细胞增殖及诱导细胞凋亡有关。【Objective】 To study the potential therapeutic effect on human U87MG glioma cells and the mechanism of apoptosis which induced by Anemone taipaiensis Saponin-1.【Methods】 Methyl thiazolyl tetrazolium（MTT） assay was performed to measure the effect on the proliferation of U87MG glioma cells treated with concentrations of Anemone taipaiensis Saponin-1 for 6,24,48 and 72 hours.Inverted microscopic observation,Hoechst 33258 fluorochrome stains,terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end labeling（TUNEL） and flow cytometry were used to observe the morphologic changes and the apoptosis.Western blotting was performed to detect the expression of surviving and caspase-3 in U87MG cells.【Results】 Anemone taipaiensis Saponin-1 significantly inhibited the cell viability of U87MG glioma cells,Western-blotting showed that Anemone taipaiensis Saponin-1 down-regulate the expression of anti-apop-tosic protein surviving and activated the pro-apoptotic protein caspase-3.【Conclusion】 In vitro studies showed,Anemone taipaiensis Saponin-1 could effectively inhibit proliferation of U87MG glioma cells and in duce them apoptosis.The mechanism of inhibiting cell proliferation may related to inducing the apoptosis.

关 键 词：太白银莲花 皂甙-1 胶质瘤U87MG 凋亡 

分 类 号：R730.2[医药卫生—肿瘤] Q253[医药卫生—临床医学]