鞣花酸抗肝癌作用的相关分子  

Related molecules of ellagic acid anti-hepatoma

作　　者：王建红[1] 赵宁[1] 田晶[1] 范才文[1] 罗琴[1] 潘红杰[1] 向秋[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《中国现代医学杂志》2013年第11期36-39,共4页China Journal of Modern Medicine

基　　金：广西自然科学基金(No:2010GXNSFC013024);广西壮族自治区中医药管理局资助项目(No:GZKZ09-72);广西壮族自治区教育厅项目(No:200911MS166);教育部回国基金

摘　　要：目的探索五倍子提取物鞣花酸抗肝癌生物学活性的相关分子。方法鞣花酸体外孵育肝癌HepG-2细胞,采用细胞形态学、四甲基偶氮唑盐(MTT)比色法和Hoechst33258染色分析细胞的增殖与凋亡;West-ern-blotting分析基因表达。结果鞣花酸剂量依赖性抑制HepG-2细胞增殖,诱导其凋亡;鞣花酸抑制肿瘤相关基因CtBP、Stathmin和COX-2的表达,随鞣花酸浓度升高,抑制作用增强。结论鞣花酸通过抑制CtBP、Stathmin和COX-2的表达发挥抗肝癌的生物学作用。【Objective】 To explore the molecules associated with biological activity of gallnut extract ellagic acid anti-hepatoma.【Methods】 Hepatoma cell line was incubated with ellagic acid in vitro.Cell proliferation,apoptosis and gene expression were analyzed with cell morphology,four methyl thiazolyl tetrazolium（MTT） assay,Hoechst 33258 dyeing and western blotting.【Results】 Ellagic acid inhibited the proliferation of HepG-2 cells and induced the apoptosis of it dose-dependently.Ellagic acid inhibited the expression of tumor associated genes CtBP,stathmin and COX-2.As ellagic acid dose increased,inhibition of gene expression was enhanced.【Conclusions】 Ellagic acid takes anti-hepatocarcinoma biological effect by inhibiting the expression of CtBP,stathmin and COX-2.

关 键 词：鞣花酸 肝癌 增殖 COX-2 CTBP 

分 类 号：R735.7[医药卫生—肿瘤]