固相萃取分离甘油三酯水解产物中的单甘酯  

Separation of the monoglycerides in triglyceride hydrolysis products with SPE technology

作　　者：李兴峰[1] 张志国[2] 

机构地区：[1]河北科技大学生物科学与工程学院,石家庄050018 [2]内蒙古蒙牛乳业(集团)股份有限公司研发中心,呼和浩特011500

出　　处：《食品科技》2013年第7期60-62,66,共4页Food Science and Technology

基　　金：国家"十二五"高技术研究发展计划项目(2012AA10160504;2011AA100901)

摘　　要：甘油三酯水解产物中的单甘酯和食品生产添加的乳化剂中的单甘酯的分离通常采用薄层色谱法。研究基于氨基柱的分离原理,采用氨基固相萃取柱分离、纯化甘油三酯水解产物中的单甘酯,通过优化淋洗液、洗脱液的条件,单甘酯能够从甘油三酯、甘油二酯中完全分离出来,用该方法对甘油三酯、甘油二酯、单甘酯标准品和食品用乳化剂中单甘酯完成分离,相对于薄层色谱法简化了操作步骤,提高了分离结果的准确度。The monoglyceride of triglyceride hydrolysis products and food emulsifying agent usually was separated by thin layer chromatography. Based on the separation principle of amino column, amino solid- phase extraction column was used as purification of monoglyceride of triglyceride hydrolysis products. Monoglyceride can be completely separated from the triglycerides and diglyceride through the optimization of eluent solution and elution solution. The monoglyceride of the standard of triglyceride, diglyceride, monoglyceride and food emulsifier were completely separated using the method above. Relative to the thin layer chromatographic method, the method of SPE simplified the operation steps, and improves the accuracy of the separation results.

关 键 词：固相萃取 薄层色谱法 单甘酯 甘油三酯 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]