In vitro assay of cytoskeleton nanomechanics as a tool for screening potential anticancer effects of natural plant extract, tubeimoside I on human hepatoma (HepG2) cells  

作　　者：ZHAO HongBo WANG YaShu JIANG XueMei SHI XiaoHao ZHONG HongZhe WANG YaJie CHEN Jun DENG LinHong 

机构地区：[1]Institute of Biomedical Engineering & Health Sciences, Changzhou University [2]Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering College, Chongqing University [3]Xinjiang Provincial Corps Hospital, Chinese People's Armed Police Forces [4]Liaoning Provincial Blood Center [5]The 324th Hospital of the Chinese People's Liberation Army

出　　处：《Chinese Science Bulletin》2013年第21期2576-2583,共8页

基　　金：supported by the National Natural Science Foundation of China (11172340);Training Program for Hundreds of Distinguished Leading Scientists of Chongqing;Chongqing Natural Science Foundation (CSTC2010BA5001 and CSTC2012JJA0588);Fundamental Research Funds for the Central Universities (CQDXWL-2012-123)

摘　　要：Cytoskeleton nanomechanics characterizes cancer cell's physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I (TBMS I) on human hepatoma (HepG2) cells by using optical magnetic twisting cytometry, a well-established technique for measuring nanomechanics of the Factin cytoskeleton. TBMS I is a natural compound extracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness (G) of HepG2 cells was affected by TBMS I. G′ exhibited a typical power law with respect to the loading frequency (f), i.e. G^f . The magnitude of G′ and the value of exponent (α) of the HepG2 cells decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver (L-02) cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of Factin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candidates.Cytoskeleton nanomechanics characterizes cancer cell＇s physical behaviors such as how it spread and invade. For anticancer drug, cytoskeleton nanomechanics may be a target to inhibit invasiveness and metastasis of cancer cells. Therefore, in vitro assay of cytoskeleton nanomechanics may be used to evaluate the effects of potential anticancer drug on various cancer types. Here, we investigated the effects of tubeimoside I （TBMS I） on human hepatoma （HepG2） cells by using optical magnetic twisting cytome- try, a well-established technique for measuring nanomechanics of the F-actin cytoskeleton. TBMS I is a natural compound ex- tracted from a traditional Chinese herbal medicine, and is reported with antitumor effect. In this study, we demonstrated that the cytoskeleton stiffness （G＇） of HepG2 cells was affected by TBMS I. G＇ exhibited a typical power law with respect to the loading frequency （f）, i.e. G-f^a. The magnitude of G＇ and the value of exponent （a） of the HepG2 ceils decreased consistently with the increase of concentration for TBMS I exposure. In addition, the HepG2 cells responded to TBMS I much faster than the normal liver （L-02） cells. Such alteration of cytoskeleton nanomechanics induced by TBMS I was reported for the first time, which was further corroborated by assays of F-actin cytoskeleton structure and cell migration. Taken together, these results suggest that in vitro assay of cytoskeleton nanomechanics may have a great potential as an additional tool in screening of anticancer drug candi- dates.

关 键 词：肌动蛋白细胞骨架 HepG2细胞 天然植物提取物 人肝癌细胞 纳米力学 体外试验 抗癌作用 药物筛选 

分 类 号：R735.7[医药卫生—肿瘤]