鼻咽癌VEGF-C和Flt-4的表达与淋巴管生成及淋巴结转移的相关性  

Expression of VEGF-C and its receptor Flt-4 in nasopharyngeal carcinoma and their relationship with lymph node metastasis

作　　者：李文光[1] 张娟[2] 陈永平[2] 戴志杰[1] 

机构地区：[1]深圳市第五人民医院耳鼻咽喉科,广东深圳518001 [2]深圳市第五人民医院病理科,广东深圳518001

出　　处：《中国耳鼻咽喉颅底外科杂志》2013年第3期196-198,203,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：深圳市2010年科技计划项目(201003427);深圳市罗湖区2011年软科学研究计划立项

摘　　要：目的探讨血管表皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)及其受体酪氨酸激酶-4(fsm-like tyrosine kinases-4,Flt-4)在鼻咽癌中的表达,同时探讨其淋巴管生成与鼻咽癌淋巴结转移的关系。方法采用免疫组化法检测80例鼻咽癌组织及62例正常对照组织中VEGF-C、Flt-4的表达,计数各组表达的阳性脉管数,并分析其与临床表现和淋巴转移的关系。结果鼻咽癌组织中VEGF-C、Flt-4的阳性表达率显著高于正常对照组(P<0.05);有淋巴结转移组的VEGF-C阳性率显著高于未发现淋巴结转移组(87.23%vs 54.55%,P<0.05);有淋巴结转移组的Flt-4的蛋白表达阳性脉管数显著高于未发现淋巴结转移组(18.55±4.54 vs 10.75±2.63,P<0.05)。结论 VEGF-C的高表达引起肿瘤淋巴管生成增多,可能促进鼻咽癌更广泛的淋巴结转移。Objective To investigate the relationship between the expression of vascular endothelial growth faetor （VEGF-C）, Flt-4 and lymph node metastasis in nasopharyngeal eareinoma （NPC）. Methods Immunohistochemical staining （SP） was used for detecting the expression of VEGF-C and Flt-4 in 80 specimens of NPC and 62 of normal tissues. The microvessel density was counted in all specimens. Their relationship between tumor＇s clinicopathologic eharacteristics and lymph node metastasis was analyzed. Results The positive rates of VEGF-C and Fh-4 in NPC were significantly higher than those in the control group （P 〈 0.05 ）. The positive rate of VEGF-C in NPC specimens with lymph node metastasis was obviously higher than that withno lymph node metastasis （ P 〈 0. 05 ） . The vessel count with positive Fh-4 in NPC specimens with lymph node metastasis was also higher than that in specimens withno lymph node metastasis （ P 〈 0.05 ）. Conclusions VEGF-C and Flt-4 might promote lymphatic metastasis by inducing lymphangiogenesis in NPC tissue.

关 键 词：鼻咽癌 血管表皮生长因子-C 酪氨酸激酶-4 淋巴管 淋巴结转移 

分 类 号：R739.9[医药卫生—肿瘤]