小干扰RNA沉默KIAA0101的表达对皮肤鳞状细胞癌SCL-1细胞增殖和迁移的影响  

Effect of small interfering RNA-induced silencing of KIAA0101 gene expression on the proliferation and invasion of a cutaneous squamous cell carcinoma cell line SCL-1

作　　者：李敏[1] 夏永华[1] 刘冬[1] 付丹丹[1] 李占国[1] 田中伟[1] 

机构地区：[1]新乡医学院第一附属医院皮肤科,河南卫辉453100

出　　处：《中华皮肤科杂志》2013年第7期489-491,共3页Chinese Journal of Dermatology

基　　金：河南省科技厅自然科学基金（114300510014）;河南省教育厅科学技术研究重点项目（13A320852）;河南省教育厅自然科学研究计划项目（2011A320017）;新乡医学院重点研究领域招标课题（zD2011-25）

摘　　要：目的探讨KIAA0101蛋白表达下调对皮肤鳞状细胞癌（SCC）SCL-1细胞增殖和细胞侵袭能力影响的分子机制。方法将KIAA0101siRNA和对照siRNA分别转染SCL-1细胞，将SCL-1细胞分为3组：未转染组、对照siRNA组和KIAA0101siRNA组。Western印迹检测3组细胞中KIAA0101蛋白的表达，CCK-8试剂检测细胞增殖，用Boyden小室检测细胞侵袭能力，Westem印迹检测细胞增殖和细胞侵袭相关蛋白的表达。结果KIAA0101siRNA组中KIAA0101蛋白的相对表达量为0．062±0．095，显著低于未转染组（0．359±0．044）和对照siRNA组（0．379±0．025），P〈0．05，SCL-1细胞的增殖和侵袭能力亦降低（P〈0．05）。此外，与未转染组和siRNA对照组相比，KIAA0101siRNA组中p21蛋白表达显著上升，而基质金属蛋白酶2表达显著下调（P〈0．05）。结论KIAA0101表达下调介导的SCL-1细胞增殖抑制和侵袭能力降低与p21和基质金属蛋白酶2表达变化相关。Objective To investigate the effect of downregulation of KIAA0101 protein expression on the proliferation and invasion of a cutaneous squamous cell carcinoma cell line SCL-1, and to explore possible molecular mechanisms underlying the effect. Methods SCL-1 cells were classified into three groups： siRNA control group transfected with the control siRNA, KIAAO101 group transfected with KIAA0101 siRNA, and untreated group remaining untreated. After additional culture, Western blot was used to detect the expression of KIAAOIO1 protein and proteins associated with cell proliferation and invasion, cell counting kit-8 （CCK-8） to evaluate cellular proliferative activity, and Boyden chamber assay to estimate invasive ability of cells. Results The relative expression level of KIAA0101 protein was 0.062 ± 0.095 in the KIAA0101 group, significantly lower than that in the untreated group （0.359 ± 0.044, P 〈 0.05） and siRNA control group （0.379 ± 0.025, P 〈 0.05）. A significant decrease was observed in cellular proliferative activity （from 24 to 96 hours） and invasive activity （at 48 hours） in the KIAAOI01 group compared with the other two groups （all P 〈 0.05）. Moreover, compared with the untreated group and siRNA control group, the KIAAO101 group showed a stronger expression of p21 protein （0.570 ± 0.060 vs. 0.048 ± 0.018 and 0.055 ± 0.014, P 〈 0.01） but a weaker expression of matrix metalloproteinase 2 （MMP2） protein （0.051 ± 0.013 vs. 0.205 ± 0.029 and 0.221 ±0.029, P 〈 0.01）. Conclusion The inhibition of SCL-1 cell proliferation and invasion induced by the downregulation of KIAA0101 gene expression may be associated with the expression changes of p21 and MMP2.

关 键 词：癌 鳞状细胞 基因 KIAA0101 RNA 小分子干扰 

分 类 号：R73[医药卫生—肿瘤]