胶原三螺旋重复蛋白1、β联蛋白和Wnt5a在黑素瘤不同阶段的表达及相关性研究  

Protein expressions of collagen triple helix repeat containing-1, β-catenin and Wnt5a in lesions from pa- tients with different stages of melanoma and their relationship

作　　者：孙兰[1,2] 王翠彦[4] 李艳秋 张凌云[1] 安湘杰[1] 王明[1] 赵云[1] SharmaGautamShuvaKant 陈思远[1] 涂亚庭[1] FurueMasutaka 黄长征[1] 郭能强[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022 [2]武汉市第一医院皮肤科 [3]华中科技大学同济医学院附属协和医院整形美容外科,武汉430022 [4]广东省惠州市中心人民医院皮肤科 [5]湖北省中山医院皮肤科 [6]日本九州大学病院皮肤科

出　　处：《中华皮肤科杂志》2013年第7期501-504,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30671891,81072232）

摘　　要：目的探讨胶原三螺旋重复蛋白1（CTHRC1）、β联蛋白和Wnt5a在色素痣和黑素瘤不同发展阶段中的表达，探讨三者在黑素瘤发生、发展过程中的可能作用及相关性。方法免疫组化SP法检测23例色素痣、14例原位黑素瘤、21例侵袭性黑素瘤和18例转移性黑素瘤组织中CTHRC1、β联蛋白和Wnt5a蛋白的表达，结合临床病理特征进行分析。结果色素痣和黑素瘤组织中CTHRC1的阳性表达率分别为34．8％、62．3％，两者比较，差异有统计学意义（P〈0．05）；β联蛋白的阳性表达率分别为21．7％、62．3％，两者比较，差异有统计学意义（P〈0．05）；Wnt5a的阳性表达率分别为34．8％、69．8％，两者比较，差异有统计学意义（P〈0．05）。CTHRCl、13联蛋白的阳性表达与黑素瘤的浸润深度和淋巴结转移相关（P〈0．05），wnt5a的阳性表达仅与淋巴结转移相关（P〈0．05）。黑素瘤中CTHRC1与β联蛋白之间以及CTHRC1与Wnt5a蛋白之间的阳性表达存在明显正相关（分别为r=0．798，P〈0．01；r=0．623，P〈0．01）。结论CTHRC1、β联蛋白和Wnt5a在黑素瘤的发生中起一定的作用，CTHRC1可能作为Wnt信号通路的辅助蛋白促进黑素瘤的发生和发展。Objective To compare the protein expressions of collagen triple helix repeat containing-1 （CTHRC1）, β-catenin and Wnt5a in lesions of nevus versus melanoma in different stages, and to assess their possible roles and relationship in the initiation and progression of melanoma. Methods Totally, 76 tissue specimens were studied in this study, including 23 cases of nevus, 14 cases of melanoma in situ, 21 cases of invasive melanoma and 18 cases of metastatic melanoma. Immunohistochemical SP method was used to detect the protein expressions of CTHRC1, β-catenin and Wnt5a in these tissue specimens. The clinical and pathological implications of these protein expressions were assessed. Results The expression rate in nevus and melanoma lesions was 34.8% and 62.3% respectively for CTHRC1 （P 〈 0.05）, 21.7% and 62.3% respectively for β-catenin （P 〈 0.05）, 34.8% and 59.8% respectively for Wnt5a （P 〈 0.05）. The expressions of CTHRC1 and β-catenin were associated with the invasion depth and lymph node metastasis of melanoma （both P 〈 0.05）, and the expression of Wnt5a was only related to the lymph node metastasis of melanoma （P 〈 0.05）. There was a positive correlation between the protein expression of CTHRC1 and β-catenin as well as between that of CTHRC1 and Wnt5a （r = 0.798, 0.623, respectively, both P 〈 0.01）. Conclusions CTHRC1,β-catenin and Wnt5a appear to play a certain role in the development of melanoma, and CTHRC 1 may serve as a cofactor of the Wnt signaling pathway in promoting the initiation and progression of melanoma.

关 键 词：黑色素瘤 痣 胶原三螺旋重复蛋白1 Β连环素 WNT5A 

分 类 号：R739.5[医药卫生—肿瘤]