Cul5基因抑制卵巢癌细胞生长  

Effect of Cullin5 on the Growth of Ovarian Cancer Cells

作　　者：王艳丽[1] 刘尚[1] 刘民[1] 汤华[1] 

机构地区：[1]天津医科大学基础医学院 天津市生命中心实验室,天津市300070

出　　处：《医学分子生物学杂志》2013年第3期131-135,共5页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.31270818,31071191,31101000）,天津市自然科学基金（No.2JCZDJC25100）

摘　　要：目的观察cullin5（cul5）基因对OVCAR3细胞生长活性和增殖的影响。方法构建cul5的过表达和敲降质粒。用Western印迹检测质粒的有效性。用MTF和集落形成实验检测cul5对OVCAR3细胞增殖的影响。结果酶切和测序鉴定表明质粒构建成功。实验表明，过表达cul5能够抑制OVCAR3细胞的生长活性和集落形成率，敲降cul5能够增强OVCAR3细胞的生长活性和集落形成率。结论cul5在卵巢癌中起到抑癌基因的作用。Objective Objective To investigate the effect of cullin5 （culS） on cell viability and proliferation of OVCAR3 cells. Methods The plasmids that could overexpress and knockdown cul5 protein were constructed, respectively. Western blotting was used to check the validity of plasraids. MTT and colony formation were used to detect the effects of cul5 on OVCAR3 cell viability and proliferation. Results All constructs were verified by restriction enzyme digestion and sequencing. It was shown that overexpression of cul5 in OVCAR3 cells reduced the cell viability and colony forma- tion rate; knockdown of cul5 in OVCAR3 cells increased cell viability and colony formation rate. Conclusion cul5 acts as tumor suppressor in ovarian cancer.

关 键 词：cul5基因 卵巢癌 MTT 集落形成 

分 类 号：R737.31[医药卫生—肿瘤]