基于核糖体基因的鳞钙皮菌种内分子关系分析  

Molecular analysis of Didymium squamulosum based on ribosomal gene sequences

作　　者：李姝[1] 王琦[1] 李玉[1] 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118

出　　处：《菌物学报》2013年第4期764-770,共7页Mycosystema

基　　金：国家自然科学基金(No.71070012)

摘　　要：为探讨不同地域的鳞钙皮菌Didymium squamulosum种内分子亲缘关系,通过PCR扩增鳞钙皮菌子实体及原质团DNA,得到SSU、ITS1-5.8S-ITS2 rRNA基因区域,并以SSU、5.8S rRNA基因片段构建NJ亲缘关系树。DNA of Didymium squamulosum was extracted from sporophores and plasmodia. SSU and ITS1-5.8S-ITS2 rRNA sequences from sporangia and plasmodia were amplified and sequenced. The molecular genetic relationships of D. squamulosum from different geographical regions were analyzed based on SSU and 5.8S rRNA sequences using neighbor-joining method.

关 键 词：黏菌 SSU RRNA ITS基因区域 5 8S rRNA基因片段 

分 类 号：Q949.31[生物学—植物学]