MicroRNA-155对喉鳞状细胞癌Hep-2细胞株的作用  

Effect of MicroRNA-155 on laryngeal squamous cell carcinoma Hep-2 cells

作　　者：王洋[1] 王斌全[1] 高伟[1] 张春明[1] 皇甫辉[1] 温树信[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉头颈外科山西省耳鼻咽喉研究所,山西太原030001

出　　处：《中国耳鼻咽喉头颈外科》2013年第6期285-289,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(81172584)

摘　　要：目的探讨microRNA-155(miR-155)过表达对喉鳞状细胞癌(简称喉鳞癌)Hep-2细胞恶性生物学表型的影响。方法将miR-155模拟物(miR-155 mimics)转染至Hep-2细胞,qRT-PCR检测转染前后miR-155表达水平,CCK-8法和平板克隆形成实验评价细胞增殖能力,流式细胞术及Hoechst33342-PI双染法检测细胞凋亡,划痕试验评价细胞迁移能力。结果转染miR-155mimics后其表达水平明显上调,Hep-2细胞增殖能力增强,细胞的凋亡和坏死比例明显下降,划痕试验结果示过表达miR-155的Hep-2细胞展现了更好的迁移愈合能力。结论过表达miR-155可以促进喉鳞癌Hep-2细胞的增殖与迁移,抑制其凋亡坏死,可能在喉鳞癌的发生发展中通过多种途径作为癌基因发挥功能。OBJECTIVE To investigate the effect of microRNA-155 （miR-155） over-expression on malignant phenotype in Hep-2 cell line of laryngeal squamous cell carcinoma. METHODS miR-155 mimics were transfected into Hep-2 cells by Lipofectamine RNAiMAX. miR-155 expression level was detected by qRT-PCR. Cell proliferation were evaluated by CCK- 8 assay and colony formation. Cell apoptosis were analyzed by flow cytometry and Hoechst 33342 ＆ propidium iodide staining. Cell migration was detected by wound healing assay. RESULTS After transfection of miR-155 mimics into Hep-2 cells, the data of qRT- PCR showed that miR-155 expression level was significantly up-regulated. CCK-8 assay and colony formation revealed that growth or proliferation ability of miR-155 group was obviously promoted.Cell apoptosis or death was suppressed after over-expressed miR- 155 which was analyzed by flow cytometry and Hoechst 33342＆ propidium iodide staining. Remarkable enhancement of cell migration was observed in miR- 155 group which was detected by wound healing assay. CONCLUSION Over-expression of miR-155 could induce proliferation and migration of Hep-2 cell line, and inhibit apoptosis. It might play the function as an oncogene in the development of laryngeal squamous cell carcinoma.

关 键 词：癌 鳞状细胞 细胞增殖 细胞凋亡 细胞运动 流式细胞术 MICRORNA-1 55 

分 类 号：R739.65[医药卫生—肿瘤]