金针菇单核原生质体菌株遗传差异的ISSR分析  

ISSR Analysis of Genetic Diversity of Protoplasted Mononuclear Strains of Flammulina velutipes

作　　者：王波[1] 祁丽萍[1] 鲜灵[1] 高俭[1] 贾定洪[1] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066

出　　处：《西南农业学报》2013年第3期1126-1131,共6页Southwest China Journal of Agricultural Sciences

基　　金：四川省科技厅育种攻关项目"金针菇育种材料研究与应用"[2011NZ0098-7]

摘　　要：原生质体技术在遗传分析和育种中具有重要作用。金针菇双核体在制备原生质体过程中会出现单核化。为了解单核原生质体之间是否存在差异,对3个金针菇菌株单核体原生质体不同交配型菌株以及配对而成的双核体菌株进行了ISSR分析。结果表明,3个金针菇菌株2种不同交配型单核原生质体配对的双核体与出发菌株之间遗传关系较近,遗传相似系数高的为0.97、低的为0.75;3个金针菇菌株的不同交配型菌株之间遗传差异较大,分别为0.77、0.73和0.59,表明不同交配型的单核原生质体菌株之间遗传差异显示其遗传背景的不同。Protoplast technique plays an important role in genetic analysis and breeding.Monokaryogenesis of Flammulina velutipes dikaryons probably appeared in theirs protoplast preparation.3 mononuclear strains of Flammulina velutipes of different mating types and their corresponding dikaryons strains were analyzed by ISSR to reveal the potential differences among mononuclear protoplasts.The result showed that the genetic relationships between dikaryons and their original strains were relatively close with genetic similarity coefficients between 0.75 and 0.97;the protoplasted mononuclear strains of 3 different mating types had considerable genetic diversity,and their genetic similarity coefficients were 0.77,0.73 and 0.59,respectively,which indicated their different genetic backgrounds.

关 键 词：金针菇 单核原生质体 遗传相似性 聚类分析 

分 类 号：S646.15[农业科学—蔬菜学]