漆酶对共基质体系下吲哚的去除研究  

Removal of indole by laccase in the presence of phenol

作　　者：任大军[1] 鲍欣[1] 张元元[1] 吴高明[2] 

机构地区：[1]武汉科技大学资源与环境工程学院,湖北武汉430081 [2]武汉钢铁(集团)公司安全环保部,湖北武汉430083

出　　处：《武汉科技大学学报》2013年第4期291-294,298,共5页Journal of Wuhan University of Science and Technology

基　　金：十二五国家科技支撑计划项目(2012BAC02B04);湖北省自然科学基金资助项目(2010CDB03502)

摘　　要：采用漆酶对低浓度单基质吲哚及与苯酚共存下低浓度吲哚进行去除实验。结果表明,与单基质相比,含有苯酚的体系中,漆酶对吲哚的去除效果更好;漆酶不仅可以同时去除共基质中的苯酚和吲哚,苯酚的存在还促进了漆酶对吲哚的去除;共基质体系中,苯酚浓度与吲哚的去除率有较好的线性相关性,苯酚的中间产物可能与漆酶组成了新的介体系统,促进了吲哚的氧化;与单基质相比,共基质体系中漆酶对吲哚的去除有两个较优的pH值,分别为3.5和6.5,且苯酚的加入可以减小漆酶的用量。Removals of indole by laccase from the white rot fungus Pleurotus ostreatus on condition without or with phenol were investigated. The results show that the removal rates of indole by laccase are higher in the presence of phenol. The laccase can remove both phenol and indole simultaneously, and phenol also enhances the removal of indole. There is better linear relationship between the removal rate of indole and the initial concentration of phenol in the co-substrates system. The intermediates of phenol can compose new mediator system with laccase, and this mediator enhances the oxidation of indole. The biodegradation rates of indole by laccase in the presence of phenol were higher at pH value of 3.5 and 6.5, respectively, and the addition of phenol can reduce enzyme dosage.

关 键 词：漆酶 苯酚 吲哚 氧化 

分 类 号：X172[环境科学与工程—环境科学]