影响桑树离体微枝培养的因素分析  

Factors Affecting the Vitro Micro-Branches Culture for Mulberry

作　　者：于海伟 刘琰 季晓慧[2] 史绍林[2] 戴玉伟[2] 

机构地区：[1]黑龙江省齐齐哈尔林业学校,黑龙江齐齐哈尔161006 [2]黑龙江省森林与环境科学研究院,黑龙江齐齐哈尔161005

出　　处：《防护林科技》2013年第7期31-32,共2页Protection Forest Science and Technology

摘　　要：以黑龙江省西部地区的4个桑树品系的离体微枝为外植体,建立了再生体系,从外植体的选择、培养基的选择、褐变的控制等方面分析了影响微体培养中生长状况的因子,认为采用嫩茎、选用1/2MS基本培养基,在培养中多次转接控制褐变等综合措施可实现桑微体繁殖培养。Taking four strains in vitro micro-branches of mulberry in Heilongjiang Province as explants, the regeneration system were established;factors affecting the growth in micro culture were analyzed from the selection of explants, the selection of the culture medium, the control of browning and other aspects; micropropagation cultivation for mulberry can be achieved by using tender stem, selecting 1/2MS basic medium, transferring repeatedly control browning in culture and other comprehensive measures.

关 键 词：桑 微体繁殖 褐变 培养基 

分 类 号：S888[农业科学—特种经济动物饲养]