Smo及Gli1 mRNA在食管鳞状细胞癌组织中的表达及意义  

Expression and significance of Smo and Gli1 mRNA in esophageal squamous cell carcinoma tissue

作　　者：李俊平[1] 杨建萍[1] 崔黎[1] 李晟磊[1] 赵志华[1] 陈奎生[1] 

机构地区：[1]郑州大学第一附属医院病理科,郑州450052

出　　处：《临床医学》2013年第6期1-3,F0004,共4页Clinical Medicine

基　　金：河南省科技创新杰出人才项目(114200510007)

摘　　要：目的探讨Smo及Gli1 mRNA在食管鳞状细胞癌组织中的表达及其意义。方法采用原位杂交技术检测68例食管鳞癌、35例癌旁不典型增生及68例正常食管黏膜组织中Smo及Gli1 mRNA的表达,并分析其与食管鳞癌各临床病理学参数间的关系。结果①在正常黏膜、癌旁不典型增生及食管鳞状细胞癌组织中Smo mRNA的阳性表达率依次增高,分别为0(0/38)、14.3%(5/35)和63.2%(43/68),组间两两比较差异有统计学意义(P<0.05);②Gli1 mRNA的阳性表达率在正常黏膜、癌旁不典型增生及食管鳞癌组织中亦依次增高,分别为2.6%(1/35)、17.1%(6/35)和70.6%(48/68),组间比较差异有统计学意义(P<0.05);③Smo及Gli1 mRNA的阳性表达率均与食管鳞状细胞癌组织学分级,浸润深度、淋巴结转移及TNM分期有关(P均<0.05)。结论 Smo及Gli1 mRNA过表达可能与食管鳞状细胞癌的发生、发展及浸润转移相关。Objective To investigate the expression and clinical significance of Smo and Gli1 mRNA in esophageal squamous cell carcinoma（ESCC）.Methods The expressions of Smo and Gli1 mRNA in 68 cases of ESCC tissue,35 cases of adjacent atypical hyperplasia tissue,and 68 cases of normal esophageal mucosa tissue were detected by in situ hybridization methods.The relation betweenthe expression of Smo and Gli1 mRNA and tumor clinical stages,histopathology types were analyzed.Results ①The expression rates of Smo mRNA in normal esophageal epithelium specimens,adjacenta typical hyperplasia epithelium and carcinoma were 0（0/38）,14.3%（5/35）,63.2%（43/68）,respectively.There were significant differences among the groups（P＆lt;0.05）.②The expression rates of Gli1 mRNA in normal esophageal epithelium specimens,adjacent atypical hyperplasia epithelium and carcinoma were 2.6%（1/35）,17.1%（6/35）,70.6%（48/68）,respectively.There were significant differences among the groups（P＆lt;0.05）.③The positive expression rate of Smo and Gli1 mRNA were closely correlated with the histological grade,infiltrative depth,lymphatic node metastasis and TNM grade in ESCC（P＆lt;0.05）.Conclusion The increased expression of Smo and Gli1 mRNA may be related to the genesis,development,invasion and metastasis of ESCC.

关 键 词：圆滑蛋白基因 GLI1 食管鳞状细胞癌 原位杂交 

分 类 号：R735.1[医药卫生—肿瘤]