不同病变胃组织端粒酶活性及其与幽门螺杆菌感染的关系  

The Relationship between Telomerase Activity and Helicobacter pylori Infection in Various Pathological Gastric Mucosal Lesions

作　　者：何兴祥[1] 王家龙[1] 吴捷莉[1] 袁顺玉[1] 艾莉[1] 

机构地区：[1]武汉同济医科大学附属同济医院消化内科

出　　处：《胃肠病学》2000年第1期19-22,共4页Chinese Journal of Gastroenterology

摘　　要：分析不同病变胃粘膜端粒酶活性的差异及其与幽门螺杆菌（H．pylori）感染的关系，探讨端粒酶活性、H.pylori感染与胃粘膜癌变的关系。方法：应用瑞粒重复扩增法测定正常胃粘膜、癌前病变和胃癌组织中的端粒酶活性，用酶免疫法检测H.Pylori感染患者的血清H．Pylori-CagA-IgG水平，并分析端粒酶活性与H.Pylori-CagAriIgG水平的关系。结果：172例胃镜活检标本中，正常胃粘膜、浅表性胃炎、慢性萎缩性胃炎无肠化、伴1度、2度肠化和胃癌组织的端粒酶阳性率分别为0％、0％、25％、37．5％和88．89％。45例手术切除的胃癌组织和相应的非癌胃组织也呈相似结果。正常胃粘膜、浅表性胃炎、慢性萎缩性胃炎无肠化及伴1度、2度肠化组织的H．pylori阳性率分别为0％、52.17％、60％、70％和75％。45例手术切除胃癌的非癌胃组织无肠化、伴1度、2度肠化的H．pylori感染强度分别为10．8±9．6个／50腺体、41．3±31．1个／50腺体和86．4±47．8个／50腺体。慢性浅表性胃炎患者的H、pylori-CagA-IgG抗体水平显著低于胃癌患者（P＜0.01），22例HPylori阳性胃癌患者感染的HPylori全部为cagA阳性菌株，其非癌胃粘膜有12例呈现端粒酶活性（54．55％）；相反H.pylori阳性的22例慢性浅表性胃炎患者感染的H.Pylori只有8例为cagA阳?Background/Aims: To analyze the telomerase activity implicated in Helicobacter pylori (H. pylori)infection and to evaluate the relation of telomerase activity and H. pylori infection to gastric carcinomatous changes. Methods: Telomerase activity was detected by telomere repeated amplification protocol (TRAP) in normal gastric mucosa, precancerous lesions and gastric carcinoma. Serum H. pylori-CagA-IgG antibody was determined by enzyme immunoassay in H. pylori infected patients. The relationship between telomerase activity and H. pylori-CagA-IgG antibody was studied by matched method using 22 pairs of H. pylori-positive patients including non-neoplastic mucosa surrounding the gastric cancer and that from chronic superficial gastritis (CSG). Results: The TRAP assay revealed that all normal gastric and CSG mucosa were negative for telomerase acitvity. In chronic atrophic gastritis (CAG), the positivity rates of telomerase activity of non-metaplasia, intestinal metaplasia (IM) of grade 1 and 2 were 0%, 25% and 37.5%, respectively. 16 out of l8 gastric carcinomas showed telomerase activity, and their positivity rate was the highest (88.89%). Among the 45 resected gastric specimens, the cancerous tissue and the surrounding non-neoplastic gastric mucosa showed similar results,39 out of 45 tumor revealed telomerase activity (86.67%). The positivity rates of telomerase activity of non-IM, IM grade 1 and 2 in the surrounding nontumorous tissues were 0%, 31.82% and 100%, respectively. The incidence in IM grade 2 or tumor specimens was significantly higher than that in non-IM or IM grade 1 (P< 0.01). In all biopsy specimens, the H. pylori positivity rates in normal gastric mucosa,CSG, non-IM, IM grade 1 and 2 CAG were 0%, 52.17%, 60%, 70% and 75%, respectively. In 45 resected gastric carcinoma, the surrounding nontumorous tissue showed similar results. This study also showed that H. pylori-CagA-IgG antibody was significantly lower in patients with CSG than that in patients with gastric carcinoma (P<0.01). All 22 H. Pylori-po

关 键 词：端粒 末端转移酶 胃肿瘤 幽门螺杆菌感染 

分 类 号：R573[医药卫生—消化系统]