MMP-9高表达在大鼠重症急性胰腺炎急性肺损伤中的作用  

High expression of MMP-9 in the pathogenesis of severe acute pancreatitis associated with acute lung injury

作　　者：鲍世韵[1] 矫元君[1] 毕建刚[1] 沈艳[1] 郭跃华[1] 

机构地区：[1]深圳市人民医院肝胆外科暨南大学第二临床医学院,深圳518020

出　　处：《国际外科学杂志》2013年第6期369-372,F0003,共5页International Journal of Surgery

摘　　要：目的 观察基质金属蛋白酶-9在大鼠重症急性胰腺炎急性肺损伤早期发病中的表达规律,并探讨其对肺损伤的影响.方法 健康成年雄性SD大鼠32只,随机分为对照组和重症急性胰腺炎3h、6h、12 h组,每组各8只,胰胆管逆行注入牛磺胆酸钠法,建立重症急性胰腺炎肺损伤大鼠模型.重症急性胰腺炎组分别在建模后3h、6h、12 h检测大鼠动脉血气、肺组织含水率、肺髓过氧化物酶活性,肺组织基质金属蛋白酶-9活性,光镜下进行胰腺和肺病理损伤评分.免疫组化法检测基质金属蛋白酶-9的表达,结果应用Image-Pro Plus专业图像分析系统定量分析.对照组在术后12 h检测上述相关指标.结果 造模成功后,3h肺组织基质金属蛋白酶-9开始逐渐升高,并于12 h达最高（P＜0.05）;肺损伤程度逐渐加重,肺组织含水率、髓过氧化物酶活性、PaC02逐渐升高（P＜0.05）,PaO2明显降低（P＜0.05）.结论 基质金属蛋白酶-9高表达是重症急性胰腺炎早期并发急性肺损伤的重要发病机制之一.Objective To observed the expression of matrix metalloproteinase-9 in the early onset of severe acute pancreatitis associated with acute lung injury in rats and investigate its effection in lung injury.Methods Thirty-two healthy adult male SD rats were randomly divided into two groups：Control group （n =8）,Severe acute pancreatitis group（n =24）.Severe acute pancreatitis model was induced by retrograde inject the 4％ sodium taurocholate sodium taurocholate into the biliopancreatic duct of rats.The severe acute pancreatitis group was detected the rate of lung water content、arterial blood gas.myeloperoxidase,matrix metalloproteinase-9,histopathology of the pancreas and lung injury score under the light microscope at 3 hours,6 hours and 12 hours.The matrix metalloproteinase-9 expression was detected by immunohistochemical and the results of immunohistochemical were analysed by the Image-Pro Plus image analysis system.Control group was detected the relevant indicators at 12 hours.Results Successfully modeling,the expression of matrix metalloproteinase-9 gradually increased beginning at 3 hours,at twelve hours up to the highest value（P ＜ 0.05）.The degree of lung injury,lung water content,myeloperoxidase activity,PaCO2 gradually increased（P ＜ 0.05）,PaO2 decreased significantly P ＜ 0.05）.Conclusions The high expression of matrix metalloproteinase-9 is important to the pathogenesis of severe acute pancreatitis associated with acute lung injury.

关 键 词：基质金属蛋白酶9 重症急性胰腺炎 肺损伤 发病机制 大鼠 

分 类 号：R57[医药卫生—消化系统]