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作 者:马飞[1,2] 汤俊[1] 胡传明[3] 杨家新[2] 许璞[1] 朱建一[1]
机构地区:[1]常熟理工学院生物与食品工程学院,常熟215500 [2]南京师范大学生命科学学院,南京210046 [3]江苏省海洋水产研究所,南通226007
出 处:《食品工业》2013年第7期121-124,共4页The Food Industry
基 金:海洋公益性行业专项(201105023);十二五国家支撑计划(2011BAD13B0902);常熟市科技计划项目(CS201205)
摘 要:采用顶空固相微萃取气相色谱质谱联用法(HS-SPME-GC/MS)对无氮(BG110)和含氮(BG11)条件下培养的地木耳(Nostoc commune)挥发物进行提取和测定。结果显示:无氮培养地木耳鉴定出38种化合物,主要包括烷烃(90.93%)、醇类(1.88%)、酯类(0.74%)、酮类(2.87%)、烯烃(3.22%)和其他化合物(0.9%)等;含氮培养地木耳鉴定出39种化合物,主要包括烷烃(85.24%)、醇类(5.93%)、酯类(1.98%)、酮类(1.65%)、烯烃(3.55%)和其他化合物(1.65%)等。地木耳挥发物组成为烷烃、醇类、酯类、酮类、烯烃以及其他化合物,其中烷烃含量占总挥发物的90%左右;氮源对地木耳的烷烃组成影响较大,含氮组以十七碳烷和十六碳烷为主,无氮组以十七碳烷和7-甲基-十七碳烷为主。The volatile compounds in Nostoc commune cultivated in two different conditions (with and without nitrogen in medium) is studied with headspace solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC- MS) techniques. A total of 39 types of volatile compounds, including normal paraffin hydrocarbons (90.93%), alkenes (3.22%), alcohols (1.88%), esters (0.74%), ketones (2.87%) have been identified from the Nostoc commune cultivated without nitrogen. And a total of 40 types of volatile compounds, including normal paraffin hydrocarbons (85.24%), alkenes (3.55%), alcohols (5.93%), esters (1.98%), ketones (1.65%) have been indentified from the Nostoc commune cultivated with nitrogen. Normal paraffin hydrocarbons, alkenes, alcohols, esters and ketones were the main compounds of volatile compounds of Nostoc commune. Among them, the concentration of normal paraffin hydrocarbons was high to 90%. The effect of nitrogen on the composition of normal paraffin hydrocarbon was significant. The main compounds in the Nostoc commune cultivated without nitrogen were heptadecane and hexadecane, and in the one cultivated with nitrogen were heptadecane and 7-methyl-heptadecane.
关 键 词:地木耳 氮源 挥发物 气相色谱-质谱联用法
分 类 号:TS255.1[轻工技术与工程—农产品加工及贮藏工程]
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