凝胶色谱净化-高效液相色谱法测定餐饮用油中苯并(a)芘  

Determination of benzo (a) pyrene in the food with oil by gel purification of condensed solvent conversion and high-performance liquid chromatography

作　　者：崔鹏[1] 吕东晋[1] 

机构地区：[1]西宁市疾病预防控制中心,青海西宁810007

出　　处：《现代预防医学》2013年第14期2605-2607,共3页Modern Preventive Medicine

摘　　要：目的建立餐饮用油脂中苯并(a)芘的测定方法。方法 BaP经乙酸乙酯+环己烷(1:1)提取,经凝胶色谱(GPC)净化,容积转换浓缩后,采用高效液相色谱法C18柱(5μm,4·6mm×250mm),流动相为乙腈-水(95+5),流速1.0ml/min,激发波长290nm,发射波长430nm进行检测,外标法定量。结果 BaP标准曲线在1.0～10.0μg/L范围内呈线性关系,r=0.999,回收率为88.0%～95.0%,RSD均小于4%,方法检出限0.1μg/kg。结论该测定方法具有前处理简单、快速、准确、灵敏度高等优点,可适用于餐饮用油脂样品BaP的测定。OBJECTIVE To establish the method of determination benzo （a） pyrene （BaP） in the food with oil. METH- ODS BaP was extracted by the ethyl acetate and cyclohexane （1： 1） and purified by gel permeation chromatography （GPC）. After the volume conversion concentrated, the high-performance liquid chromatography C18 column （5μm, 4.6mm×250mm） was used, mobile phase of acetonitrile - water （95 ＋ 5）, 1.0ml / min flow rate, 290nm excitation wavelength, 430nm emission wavelength and then the external standard for quantization was used for detection. RESULTS BaP standard curve in 1.0-10.0μg / L range was linear, r = 0.999, recovery rate was 88.0% -95.0% , RSD was less than 4% and the detection limit 0.1μg / kg. CONCLUSION The method is simple, fast, accurate, high sensitivity and suitable for the determination of BaP sample of the food with oil.

关 键 词：苯并(A)芘 凝胶渗透色谱 溶剂转换 高效液相色谱 餐饮用油脂 

分 类 号：R155.58[医药卫生—营养与食品卫生学]