胰腺癌细胞株和胰腺癌组织中miRNAs的表达检测  

The Expression Level of miRNAs in Pancreatic Cancer Cell Lines and Pancreatic Cancer Tissues

作　　者：艾福录[1] 华向东[1] 刘也夫[1] 林杰[1] 郝志强[1] 刘梦颖[2] 

机构地区：[1]辽宁省肿瘤医院肝胆胰脾外科,辽宁沈阳110042 [2]第三军医大学临床医学系,重庆400038

出　　处：《现代生物医学进展》2013年第17期3238-3242,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30872464)

摘　　要：目的:探明miRNAs在胰腺癌细胞株及组织中的表达情况,证实miRNAs的差异表达与胰腺癌的发生有相关性。方法:对胰腺癌细胞株和胰腺癌组织进行总RNA的提取,通过Real-time PCR方法检测17种miRNAs(miR-16、miR-20a、miR-21、miR-26a、miR-142-3p、miR-155、miR-210、miR-181a、miR-181b、miR-196a、miR-939、miR-223、miR-1202、miR-1207-5p、miR-1825、miR-1228、miR-1915)在胰腺癌细胞株及胰腺癌组织中的表达,分析miRNAs的表达与胰腺癌患者临床表现之间有无相关性。结果:胰腺癌细胞株和胰腺癌组织中miRNAs的表达明显较正常胰腺组织高。癌组织及癌旁组织中miRNAs表达量在不同性别的胰腺癌患者中差异不大(P>0.05),并且miRNAs的表达与患者年龄及其血清CA19-9关系不大。结论:经筛选的miRNAs可以作为胰腺癌的诊断标志。胰腺癌组织中的miRNAs表达并不是一成不变的,而是随着肿瘤的生长而不断发生变化。Objective： To investigate the relative abundances of those miRNAs screened by microarray in pancreatic cancer cell lines and in pancreatic cancer tissues and confirm the correlations between those miRNAs and pancreatic cancer.Methods： The total RNA were extracted from pancreatic cancer cell lines and pancreatic tissue.The 17 miRNAs（miR-16,miR-20a,miR-21,miR-26a,miR-142-3p,miR-155,miR-210,miR-181a,miR-181b,miR-196a,miR-939,miR-223,miR-1202,miR-1207-5p,miR-1825,miR-1228,miR-1915） were detected by Real-time PCR.And then we can analyze the relationship between miRNAs and clinical characteristics of patients with pancreatic cancer.Results： Compared with normal pancreatic tissue,the expression of miRNAs in pancreatic cancer cell lines and the pancreatic cancer tissue were significantly increased.Amount of miRNAs expression in carcinoma tissues and adjacent tissues in patients with pancreatic cancer has no statistical difference in different genders,and it has little correlation with the age and the level of serum CA19-9.Conclusion： The screened miRNAs can be as potential diagnostic markers of pancreatic cancer.MiR-223 expression level was negatively correlated with the maximum tumor diameter and tumor stage,which suggested that miRNAs may change with the occurrence and progress of pancreatic cancer.

关 键 词：胰腺癌 MICRORNAS 诊断 标志物 

分 类 号：R735.9[医药卫生—肿瘤]