环氧合酶基因多态性与阿司匹林抵抗的关系  

Correlation of Aspirin Resistance with Gene Polymorphisms of Cyclooxygenase

作　　者：黄洁杰[1] 梁辉[2] 王文安[2] 周熙琳[2] 窦存芳[2] 

机构地区：[1]上海交通大学医学院附属新华医院血液内科,上海200092 [2]上海交通大学医学院附属新华医院崇明分院老年科,上海202150

出　　处：《现代生物医学进展》2013年第17期3258-3262,共5页Progress in Modern Biomedicine

基　　金：上海市崇明县科委基金项目(CK2011-31)

摘　　要：目的:研究缺血性脑卒中患者阿司匹林抵抗与血小板环氧合酶(COX)单核苷酸基因多态性(SNP)的相关性。方法:169位缺血性脑卒中患者服用阿司匹林100 mg/d至少7 d,根据血栓弹力图(TEG)检测结果分为阿司匹林敏感(AS)组和非阿司匹林敏感(NAS)组,并采用PCR产物直接测序分析法对患者的COX-1(-1676A>G)、COX-2(-765G>C)基因的单核苷酸多态性进行分析。结果:根据TEG检测结果,非阿司匹林敏感(NAS)组66例,阿司匹林敏感(AS)组103例。两组患者间COX-1基因-1676A>G SNP位点的基因型和等位基因频率分布差异具有统计学意义(P=0.016和P=0.008),特别是其等位基因频率分布具有显著差异。两组患者间COX-2基因-765G>C SNP位点的基因型和等位基因频率分布差异无统计学意义(P=0.084和P=0.090)。结论:COX-1基因-1676A>G位点多态性可能与缺血性脑卒中患者患者阿司匹林抵抗的发生相关,COX-2基因-765G>C位点多态性可能与阿司匹林抵抗的发生无关。Objective： To explore the relationship between COX-1-1676AG,COX-2-765GC single nucleotide polymorphisms（SNP） and aspirin resistance in patients with Ischemic stroke.Methods： One hundred and sixty-nine patients with Ischemic stroke taking 100 mg aspirin at least 7 days were selected and divided into the aspirin sensitive group（AS） and non-aspirin sensitive group（NAS） according to thrombelastography（TEG）.In these patients,COX-1-1676AG,COX-2-765GC gene polymorphisms were analyzed by polymerase chain reaction and DNA sequencing assay.Results： Patients were divided into NAS（66 cases） and AS（103 cases）.There were significant differences in the genotype distribution and allele frequency of COX-1-1676AG SNP between two groups（P=0.016 and P=0.008）.There were no significant difference in the genotype distribution and allele frequency of COX-2-765GC SNP between two groups（P=0.084 and P=0.090）.Conclusion： In the patients with ischemic stroke,COX-1-1676AG polymorphism may be related to the aspirin resistance;however,COX-2-765GC polymorphism was not associated with the aspirin resistance.

关 键 词：缺血性脑卒中 阿司匹林抵抗 血栓弹力图 基因多态性 环氧合酶 

分 类 号：R743[医药卫生—神经病学与精神病学]