MicroRNA-34a通过多向调控促癌基因抑制肾癌细胞生长  

MicroRNA-34a Inhibits Cell Growth by Regulating Many Oncogenes in Renal Cell Carcinoma Cells

作　　者：张长存[1] 周立斌[1] 莫仁[1] 刘勇超[1] 凡杰[1] 

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,上海200080

出　　处：《现代生物医学进展》2013年第18期3445-3447,3460,共4页Progress in Modern Biomedicine

基　　金：上海市教育委员会科研创新项目(重点项目)(09ZZ25);上海市科学技术委员会医学引导项目(09411968110)

摘　　要：目的:探讨microRNA-34a(miR-34a)在肾癌细胞中的生物学作用及调控机制。方法:应用miR-34amimics在体外转染769P,786-O和Caki-1细胞;运用qRT-PCR检测miR-34a在三个细胞株的相对表达情况,以及转染后癌基因mRNA的表达情况;观察miR-34a对细胞生长的影响。结果:769P,786-O和Caki-1细胞中miR-34a在786-O中表达最低,769P次之,Caki-1表达最高;利用miR-34a mimics升高769P,786-O和Caki-1细胞miR-34a,发现三个细胞株多个癌基因mRNA表达不同程度的降低(P<0.05)及生长和集聚能力的降低。结论:miR-34a可能通过调控多个癌基因表达在肾癌中起抑癌作用。miR-34a mimics可抑制肾癌细胞的生长,因此miR-34a有可能作为肾癌基因治疗的新靶点。Objective：To explore the mechanism and function of microRNA-34a（miR-34a） in renal cell carcinoma cells.Methods：Renal cell carcinoma cell lines of（769P,786-O and Caki-1） were transfected with miR-34a mimics and negative control.Then,the relative expression of miR-34a in three renal cell carcinoma cell lines and the mRNA expression of multiple oncogenes after transfection were examined by quantitative real-time PCR.The cell growth and colony formation ability between the groups of miR-34a mimics and negative control were observed.Results：The expression of miR-34a was highest in Caki-1 and lowest in 786-O.Overexpression of miR-34a down-regulated the mRNA expression of multiple oncogenes in different degrees in three renal cell carcinoma cell lines.The ability of cell growth and cell colony formation were also suppressed after the expression of miR-34a was elevated in renal cell carcinoma cell lines.Conclusion：miR-34a can inhibit cell growth by down-regulating multiple oncogenes,and thus may be a new target in the treatment of renal cell carcinoma.

关 键 词：MicroRNA-34a 癌基因 肾癌 肾癌细胞 细胞生长 

分 类 号：R730.231[医药卫生—肿瘤] R737.11[医药卫生—临床医学]