竹节参总皂苷不同提取工艺的比较研究  

Comparative Study on Total Saponins of Japanese Ginseng in Different Extraction Technology

作　　者：张海滨[1] 张长城[1] 何毓敏[1] 关乔中[2] 毛帅[2] 孙志伟[1] 崔倩倩[1] 袁丁[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学化学与生命科学学院,湖北宜昌443002

出　　处：《现代生物医学进展》2013年第18期3457-3460,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31070314)

摘　　要：目的:通过比较竹节参总皂苷不同提取工艺以优选竹节参总皂苷稳定、可靠的提取工艺,以期为民族中药竹节参的现代化开发利用奠定实验基础。方法:以提取物中竹节参总皂苷含量和齐墩果酸含量以及提取物抗氧化能力作为考察指标,比较60%乙醇回流提取法、水提醇沉法、泡沫分离法、水饱和正丁醇超声提取法、60%乙醇超声提取法等提取工艺,希望为竹节参总皂苷提取工艺的选择提供参考。结果:60%乙醇超声提取法所得竹节参总皂苷提取率、齐墩果酸转移率相对较高,分别达到99.31%和33.59%,且提取物体外抗氧化活性相对较高达到71.30 U/g。结论:60%乙醇超声提取法具有提取时间短,稳定性好,有效成分转移率高,提取成本较低,可广泛应用于大型工业化生产等优点,为竹节参总皂苷的较优提取工艺。Objective：To investigate the optimal extraction process of total saponins from panax japonicus.Method：The yield of total saponins,oleanolic acid content and anti-oxidant activity in vitro from the extraction were used as index to optimize the condition of the extraction process within 60% ethanol reflux extraction,water extraction and alcohol sink method,foam fractionation,water saturation n-buoh the ultrasonic extraction method and 60 % ethanol ultrasonic extraction method.Results：60 % ethanol ultrasonic extraction was the best method with total saponins extraction yield up to 24.29 %（total saponins content 88.85 %）,and oleanolic acid transfer rate（33.59%） and anti-oxidant activity（71.30%） were all relatively high,compared with other methods.Conclusion：The 60% ethanol ultrasonic extraction process is relatively simple,fast,efficient and economical,with high transfer rate of effective component,so it could be used for extracting panax japonicas.

关 键 词：竹节参 总皂苷 提取工艺 齐墩果酸 体外抗氧化 

分 类 号：R284[医药卫生—中药学]