发育期锌缺乏对小鼠脑组织微管聚合作用的影响  

Effect of Dietary Zinc Deficiency on Brain Microtubule Polymerization at Developing Stage of Mice

作　　者：王福俤[1] 

机构地区：[1]第二军医大学军队卫生学教研室,上海200433

出　　处：《上海铁道大学学报》2000年第5期4-7,共4页

基　　金：国家自然科学基金资助项目 ( 39770 6 43;39970 6 41);全军九五青年基金资助项目 !( 98Q 0 4 3)

摘　　要：目的　通过观察发育期锌缺乏小鼠脑组织α-微管蛋白 (α-Tub)、β-微管蛋白 (β-Tub)及微管相关蛋白 2( MAP2 )的表达情况 ,探讨膳食锌调节脑组织微管聚合作用的可能机制。方法　 80只刚怀孕 ICR母鼠随机分为严重缺锌组、轻度缺锌组、适锌组、高锌组及高锌对喂组 5组 ,在孕期和哺乳期分别饲喂含 1mg/ kg、5 mg/ kg、3 0 mg/kg、10 0 mg/ kg及 10 0 mg/ kg锌水平的实验饲料 ;断奶期 (仔鼠 2 0日龄 )后各组均改喂普通饲料。取各实验组第 70天 ( P70 ,成年 )仔鼠脑组织 ,采用 Western blot技术检测其α-Tub、β-Tub及 MAP2表达情况。结果　成年仔鼠脑组织中均有α-Tub、β-Tub及 MAP2表达 ,比较各实验组α-Tub、β-Tub及 MAP2表达水平 ,依次为 1mg/ kg组 <5 mg/ kg组 <3 0 mg/ kg组 <10 0 mg/ kg组 ,脑组织中α-Tub、β-Tub及 MAP2表达量与膳食锌水平具有明显的依赖关系。结论　发育期锌缺乏可抑制脑组织中α-Tub、β-Tub及 MAP2表达 ,而补充锌可促进其表达 ;α-Tub、β-Tub及Objective To determine the mechanism of dietary zinc deficiency on microtubule polymerization in the developing brain of mice by studying the expressions of α tubulin,β tubulin and microtubule associated protein 2.Methods Maternal ICR mice were fed with experimental diets containing different levels of zinc (1 mg,5 mg,30 mg and 100 mg zinc per kilogram of diet) during pregnancy and lactation respectively.In weaning period from 20th postnatal day (P20) to 70th day (P70),all experimental offsprings were fed with general diets.The α tubulin,β tubulin and microtubule associated protein 2 expressions in the brain on postnatal day 70 (P70,adult) were examined by Western blot assays.Results The results showed that α tubulin,β tubulin and microtubule associated protein 2 were expressed in the brain.There were closed relationships between the concentrations of dietary zinc and the levels of α tubulin,β tubulin and microtubule associated protein 2 expressions.The order of α tubulin,β tubulin and microtubule associated protein 2 levels was:1 mg/kg group<5 mg/kg group<30 mg/kg group<100 mg/kg group.Conclusion These results suggested that dietary zinc deficiency at developing stage can inhibit the expression of tubulin and microtubule associated protein 2,and zinc supplement can improve their expressions.The decline of tubulin and microtubule associated protein 2 expressions is one of the important mechanisms of microtule polymerization impairments resulting from zinc deficiency.

关 键 词：锌 微管蛋白 脑组织发育 实验营养学 小鼠 

分 类 号：R151.1[医药卫生—营养与食品卫生学]