人脐血干细胞对局灶性脑缺血大鼠神经生长因子水平及神经功能的影响  

Effect of human umbilical cord blood stem cells on nerve growth factor levels and nerve function in rats with focal cerebral ischemia

作　　者：王强[1] 汪青松[1] 张云静[2] 项倩彤[3] 刘学春[1] 黄海丽[1] 

机构地区：[1]安徽医科大学解放军临床学院神经内科,合肥230031 [2]中国药科大学,南京211198 [3]安徽医科大学基础医学院,合肥230031

出　　处：《安徽医学》2013年第1期52-55,共4页Anhui Medical Journal

基　　金：南京军区医学科技创新课题(11MA042)

摘　　要：目的探讨经静脉移植脐血干细胞治疗大鼠脑缺血模型的疗效以及对其神经功能恢复的影响。方法 LudmilaBelayev法建立大鼠大脑中动脉闭塞梗塞模型,33只大鼠随机数字表法分成生理盐水组、依达拉奉组和移植组。分别在造模1 d后经尾静脉注射生理盐水、依达拉奉和脐血干细胞进行观察。结果造模前3组血清NGF(OD值)比较差异无统计学意义(P>0.05);造模后3～40 d,依达拉奉组和脐血干细胞组血清NGF(OD值)水平下降较生理盐水组差异有统计学意义(P<0.05);而在治疗后的7、14、21、28、40 d,脐血干细胞组血清NGF(OD值)水平明显高于依达拉奉组(P<0.05)。与此同时,造模前3组神经功能评分均为0分,治疗后3组神经功能评分均有所提高,其中依达拉奉组和脐血干细胞组较生理盐水组差异有统计学意义(P<0.05);而依达拉奉组和脐血干细胞组比较,治疗后的第3、5、7 d神经功能评分差异无统计学意义(P>0.05);但治疗后的第14、21、28、40天,脐血干细胞组的神经功能评分优于依达拉奉组(P<0.05)。结论实验提示经静脉移植的脐血干细胞可迁移至大鼠局灶缺血侧脑组织,提高大鼠神经生长因子水平,并显著改善大鼠神经系统功能。Objective To explore the efficacy of the intravenous transplantation of umbilical cord blood stem cells on cerebral ischemia in rats and its nerve function.Methods Ludmila Belayev method was used to establish middle cerebral artery occlusion and reperfusion model.Thirty-three rats were randomly divided into edaravone group and transplantation group.One day after modeling the intravenous injection of saline,edaravone and umbilical cord blood stem cell line was performed respectively,and the effect was observed.Results Before modeling the serum NGF（OD value） in three groups showed no significant difference（P 0.05）;three to forty days after modeling,the decrease of serum NGF levels（OD value） in edaravone group and umbilical cord blood stem cell group showed a significant difference compared with that of the physiological saline group（P0.05）;the serum NGF（OD value） of the umbilical cord blood stem cell group was significantly higher than that of edaravone group 7,14,21,28,40 days after treatment（P0.05）.At the same time,the nerve function score of three groups before modeling was 0,while that after treatment was improved;there were significant differences in edaravone group and umbilical cord blood stem cell group compared with the saline group（P0.05）;the neurological score difference was not statistically significant between edaravone group and umbilical cord blood stem cell group 3,5 and 7 days after treatment,（P 0.05）;however,14,21,28,40 days after treatment,the neurological score of the umbilical cord blood stem cell group was better than that of edaravone group（P0.05）.Conclusion The experiments suggest that intravenous transplantation of cord blood stem cells can migrate to focal ischemic brain tissue,which improves the level of nerve growth factor in rats and the neurological function significantly.

关 键 词：脑缺血 脐血干细胞 细胞移植 神经细胞因子 

分 类 号：R743.3[医药卫生—神经病学与精神病学]